M2405

4-Methylumbelliferyl β-D-lactopyranoside

β-galactosidase substrate, ≥98.00% (TLC), powder

• Synonym(s): 4-Methylumbelliferyl β-D-lactoside
• Empirical Formula (Hill Notation): C₂₂H₂₈O₁₃
• CAS Number: 84325-23-5
• Molecular Weight: 500.45
• NACRES: NA.32
• PubChem Substance ID: 24896730
• UNSPSC Code: 12352204
• MDL number: MFCD00083406
• Beilstein/REAXYS Number: 8094402

Properties

• Product Name: 4-Methylumbelliferyl β-D-lactopyranoside, β-galactosidase substrate
• Quality Segment: 200
• assay: ≥98.00% (TLC)
• form: powder
• solubility: water: 9.80-10.20 mg/mL, clear, colorless to faintly yellow
• fluorescence: λ_ex 365 nm; λ_em 445 nm (after enzymatic degradation)
• storage temp.: −20°C
• SMILES string: CC1=CC(=O)Oc2cc(O[C@@H]3O[C@H](CO)[C@@H](O[C@@H]4O[C@H](CO)[C@H](O)[C@H](O)[C@H]4O)[C@H](O)[C@H]3O)ccc12
• InChI: 1S/C22H28O13/c1-8-4-14(25)32-11-5-9(2-3-10(8)11)31-21-19(30)17(28)20(13(7-24)34-21)35-22-18(29)16(27)15(26)12(6-23)33-22/h2-5,12-13,15-24,26-30H,6-7H2,1H3/t12-,13-,15+,16+,17-,18-,19-,20-,21-,22+/m1/s1
• InChI key: PRTGXBPFDYMIJH-KSFLKEQHSA-N

Description

Application

4-Methylumbelliferyl β-D-lactopyranoside has been used as a chromophoric substrate for cellobiohydrolase I (CBHI) in T. reesei. It has also been used as a substrate for cellobiohydrolase I (Cel7A), endoglucanase I (Cel7B) and β-glucosidase from fermented samples in enzymatic assay.

Biochem/physiol Actions

4-Methylumbelliferyl β-D-lactopyranoside is a fluorescence compound hydrolyzed by the enzyme β-glucosidase (βG) and CBH1

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable