This product is native myoglobin isolated from equine heart and is probably a mixture of oxidized myoglobin (metmyoglobin, Fe3+) and reduced myoglobin (Fe2+). The percentages of oxidized or reduced myoglobin in the product are not determined.
M1882
Myoglobin from equine heart
≥90% (SDS-PAGE), essentially salt-free, lyophilized powder
Myoglobin from equine heart
1 of 1 reviewers received a sample product or took part in a promotion
Synonym(s):
Myoglobin from horse heart
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About This Item
Form:
essentially salt-free, lyophilized powder
Assay:
≥90% (SDS-PAGE)
Biological source:
equine heart
Pricing and availability is not currently available.
biological source
equine heart
Quality Segment
assay
≥90% (SDS-PAGE)
form
essentially salt-free, lyophilized powder
Iron content
≥0.20%
technique(s)
MALDI-MS: suitable
UniProt accession no.
storage temp.
−20°C
Gene Information
horse ... MB(100054434)
Application
Myoglobin from equine heart is suitable for use in:
- spectral measurements in Beckman DU-50 or Gilford 2400 spectrophotometer[1]
- the secondary structure analysis of proteins in H2O solution using single-pass attenuated total reflection Fourier transform infrared (ATR-FT-IR) microscopy[2]
- the calibration of the mass scale at a concentration of 2 pmol/μL in Electrospray mass spectrometry[3]
- a study to investigate on-line single droplet deposition for MALDI mass spectrometry[4]
- a study to examine protein adsorption in fused-silica and polyacrylamide-coated capillaries[5]
Biochem/physiol Actions
Myoglobin is a mobile carrier of oxygen that is developed in red muscle and heart cells. This happens as a response to elevated demand for oxygen during exercise, and transports oxygen from the sarcolemma to the mitochondria of vertebrate heart and red muscle cells.[6]
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This Item | |||
|---|---|---|---|
| assay ≥90% (SDS-PAGE) | assay 95-100% | assay ≥95% (SDS-PAGE), ≥95% based on Mol. Wt. 12,384 basis | assay - |
| technique(s) MALDI-MS: suitable | technique(s) mass spectrometry (MS): suitable | technique(s) cell culture | mammalian: suitable | technique(s) - |
| biological source equine heart | biological source equine skeletal muscle | biological source horse heart | biological source bovine heart |
| form essentially salt-free, lyophilized powder | form essentially salt-free, lyophilized powder | form powder | form powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. - |
| Gene Information horse ... MB(100054434) | Gene Information horse ... MB(100054434) | Gene Information horse ... CYCS(100053958) | Gene Information - |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| M1882-1MG | 04061823206808 |
| M1882-10G | 04061834041290 |
| M1882-5G | 04061833630723 |
| M1882-250MG | 04061834041313 |
| M1882-1G | 04061834041306 |
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I would like to consult you about the valence state of the ion in MB. Is it 2+ or 3+?
1 answer-
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Was this product recombinantly expressed or was it isolated directly from the heart of the horse ?
1 answer-
This product is native myoglobin isolated from equine heart.
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Is equine myoglobin (M1882) detectable and quantifiable by common laboratory tests (ECL) for the detection of human myoglobin?
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This item has not been tested in-house for detectability and quantifiability by common laboratory tests. The specifications for this item do not include testing by these methods.
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Is Product M1882, Myoglobin from equine heart, metmyoglobin?
1 answer-
The M1882 is a mixture, but we have not analyzed the percentages of the oxidized myoglobin or the reduced myoglobin in the product.
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Is Product M1882, Myoglobin from equine heart, oxidized?
1 answer-
Yes, it is oxidized.
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Do you have the sequence for Product M1882, Myoglobin from equine heart?
1 answer-
Product M1882 - Myoglobin from equine heart is purified from equine heart. It is not sequenced.Accession P68082 for equine myoglobin has the following sequenceMGLSDGEWQQVLNVWGKVEADIAGHGQEVLIRLFTGHPETLEKFDKFKHLKTEAEMKASE DLKKHGTVVLTALGGILKKKGHHEAELKPLAQSHATKHKIPIKYLEFISDAIIHVLHSKHPGDFGADAQGAMTKALELFRNDIAAKYKELGFQGI hope that you find this information helpful.If you have further questions, please reply to this email.Sincerely,Audrey FlemingSigma-Aldrich Technical Service
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Which has more affinity for oxygen, hemoglobin or myoglobin?
1 answer-
The affinity of myoglobin for oxygen is higher than that of hemoglobin.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the molecular weight of Product M1882, Myoglobin from equine heart?
1 answer-
Based on the amino acid sequence (16,950) plus the heme group (616), the molecular weight is approximately 17,600 g/mol.
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