Viral testing is not performed on this product; only bacteriological growth is tested.
L2542
LB Broth (Miller)
Liquid microbial growth medium
1 of 1 reviewers received a sample product or took part in a promotion
Synonym(s):
Luria Broth Miller, Luria-Bertani broth, Miller
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About This Item
NACRES:
NA.85
UNSPSC Code:
41106200
Pricing and availability is not currently available.
grade
Molecular Biology
Quality Segment
sterility
sterile; 0.2 μm filtered
form
liquid
technique(s)
microbiological culture: suitable
pH
6.5-7.2
application(s)
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
General description
Miller’s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. It enables fast growth, improves growth yields for various bacterial species, and can retain the hydrophilic behavior of samples for longer periods.
Application
Features and Benefits
Miller′s LB liquid provides:
- Filter-sterilized, ready-to-use format
- Convenient package size
- Standard formulation
Other Notes
10g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract
10 g/L NaCl
5 g/L Yeast Extract
1 of 1
This Item | |||
|---|---|---|---|
| description Liquid microbial growth medium | description Highly-referenced nutrient-rich microbial growth powder medium, suitable for regular E.coli culture | description Highly-referenced nutrient-rich microbial growth powder medium with Agar, suitable for regular E.coli culture. | description Powder microbial growth medium |
| suitability nonselective for coliforms, nonselective for Escherichia coli | suitability nonselective for coliforms, nonselective for Escherichia coli | suitability nonselective for coliforms, nonselective for Escherichia coli | suitability nonselective for Escherichia coli, nonselective for coliforms |
| form liquid | form powder | form powder | form powder |
| technique(s) microbiological culture: suitable | technique(s) - | technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable |
| sterility sterile; 0.2 μm filtered | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| application(s) microbiology | application(s) food and beverages | application(s) food and beverages | application(s) food and beverages |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. room temp |
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Storage Class
12 - Non Combustible Liquids
WGK
nwg
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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is any viral testing performed on the LB medium
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Is this product defined as animal free component?
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This product is not defined as an animal-free component.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the difference among the LB - Miller products?
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LB - Miller is available in many types to suit your needs. The different product formats include powder and liquid form. The powder form is also available with agar for easy LB-agar plate preparation.L2542 (LB Miller liquid)L3522 (LB Miller powder)L3147 (LB Miller powder with agar)
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Will adding magnesium to the culture medium increase cell density?
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In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities. You may need to also increase the shaking speed of the incubator.
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What are the differences among the Luria, Lennox and Miller LB formulations?
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LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose. Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version). For some applications, even lower salt is required (Luria's low salt version).
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Which bacterial culture medium is the best choice for my application?
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Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
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