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L2270

Sigma-Aldrich

Lys-Ala 4-methoxy-β-naphthylamide dihydrochloride

dipeptidylpeptidase II substrate

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About This Item

Empirical Formula (Hill Notation):
C20H28N4O3 · 2HCl
CAS Number:
Molecular Weight:
445.38
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.28

Quality Level

Assay

≥97.5% (TLC)

form

powder

solubility

water: 50 mg/mL, clear, colorless to yellow (and colorless to faint brown and colorless to brown-yellow)

storage temp.

−20°C

SMILES string

Cl.Cl.COc1cc(NC(=O)[C@H](C)NC(=O)[C@@H](N)CCCCN)cc2ccccc12

InChI

1S/C20H28N4O3.2ClH/c1-13(23-20(26)17(22)9-5-6-10-21)19(25)24-15-11-14-7-3-4-8-16(14)18(12-15)27-2;;/h3-4,7-8,11-13,17H,5-6,9-10,21-22H2,1-2H3,(H,23,26)(H,24,25);2*1H/t13-,17-;;/m0../s1

InChI key

GJOOJLYXFGXARX-ZMQOMOPGSA-N

Application

Lys-Ala 4-methoxy-β-naphthylamide dihydrochloride has been used as a substrate to measure the dipeptidyl peptidase II (DPPII) from Vigna radiata seeds.

Substrates

Fluorogenic substrate for dipeptidyl aminopeptidase II.

Pictograms

Health hazard

Signal Word

Warning

Hazard Statements

Hazard Classifications

Carc. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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R Gossrau et al.
Histochemistry, 70(1), 53-76 (1980-01-01)
The activity of dipeptidylpeptidase II (DPP II; E.C. 3.4.14.2) was investigated by biochemical and histochemical methods in rat, mouse and guinea-pig organs as well as in human enterobiopsies. Lys-Pro-MNA and Ala-Pro-MNA showed the most favorable kinetic properties (Km, Vmax) and
F A Dolbeare et al.
Clinical chemistry, 23(8), 1485-1491 (1977-08-01)
Enzyme activity can be measured in single cells or in cell suspensions by either static or flow microfluorometry when a fluorogenic substrate is used. We have used amino acid derivatives of arylamines as fluorogenic substrates for tagging cellular proteinases. The

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