L0636

Lectin from Triticum vulgaris (wheat)

BioReagent, suitable for cell culture, lyophilized powder

• Synonym(s): WGA, Wheat germ agglutinin
• NACRES: NA.32
• UNSPSC Code: 12352202
• Form: lyophilized powder

Properties

• sterility: aseptically processed
• Quality Segment: 200
• product line: BioReagent
• form: lyophilized powder
• technique(s): cell culture | mammalian: suitable
• impurities: salt, essentially free
• storage temp.: 2-8°C

Description

General description

Lectin from Triticum vulgaris (wheat) is a dimeric glycoprotein of 36 kDa. It belongs to the family of chitin-binding proteins. Glycosylated precursor of lectin is synthesized in endoplasmic reticulum.

Application

Lectin from Triticum vulgaris (wheat) has been used:

• to conjugate with N-hydroxysuccinimide (NHS) dye
• to inhibit nuclear import
• to inhibit the nuclear pore of human cervical carcinoma HeLa and SiHa cells

Biochem/physiol Actions

Lectin from Triticum vulgaris (wheat) possessing four binding sites is specific for N-acetyl-d-glucosamine and N-acetyl-d-neuraminic acid (sialic acid) residues. Lectins also function as a fungicide. They also possess anti-nutritive effects.

WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

Preparation Note

Conjugates are prepared from affinity purified lectin.

Other Notes

Highly purified

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)