L0144

Lucifer Yellow CH dipotassium salt

Intracellular staining dye, powder

• Empirical Formula (Hill Notation): C₁₃H₉K₂N₅O₉S₂
• CAS Number: 71206-95-6
• Molecular Weight: 521.57
• NACRES: NA.77
• PubChem Substance ID: 24896249
• UNSPSC Code: 12171500
• MDL number: MFCD00013445
• Beilstein/REAXYS Number: 5224942
• Form: powder
• Quality level: 100

Properties

• Product Name: Lucifer Yellow CH dipotassium salt, powder
• form: powder
• Quality Level: 100
• color: orange
• fluorescence: λ_ex 428 nm; λ_em 540 nm
• storage temp.: room temp
• SMILES string: [K+].[K+].NNC(=O)NN1C(=O)c2cc(cc3c(N)c(cc(C1=O)c23)S([O-])(=O)=O)S([O-])(=O)=O
• InChI: 1S/C13H11N5O9S2.2K/c14-10-5-1-4(28(22,23)24)2-6-9(5)7(3-8(10)29(25,26)27)12(20)18(11(6)19)17-13(21)16-15;;/h1-3H,14-15H2,(H2,16,17,21)(H,22,23,24)(H,25,26,27);;/q;2*+1/p-2
• InChI key: HCWYSCVNSCIZCN-UHFFFAOYSA-L

Description

General description

Lucifer yellow CH is a fixable dye with an excitation and emission spectrum of 430 nm and 540 nm, respectively.LuciferYellow CH (LYCH) is a small, hydrophilic molecule, and highly fluorescent dye that is used for intracellular staining for the studies conducted on various types of cells. It acts as a marker for the detection of well-established tight junctions in smooth muscles, glia, and interstitial cells of Cajal (ICC).

Application

Lucifer yellow CH dipotassium salt has been used:

• to visualize cell morphology
• to assess intercellular communication activity through gap junction by scrape-loading assay
• as a component of the intracellular solution, which filled the dendrites for the visualization of neuronal morphology
• in the intracellular solution, to intracellularly label recorded neurons

Features and Benefits

Highly fluorescent dye, useful in marking nerve cells.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)