The molecular weight of this product has not been determined. However, IgG3 is cited as having a molecular weight of approximately 170 kDa. This value may vary due to post-translational modifications and glycolylation. The approximate molecular weight of the IgG3 heavy chain and kappa light chain are approximately 55 kDa and 23 kDa, respectively.
I5654
IgG3, Kappa from human myeloma plasma
purified immunoglobulin, >90% (microfluidic capillary gel electrophoresis)
Synonym(s):
Human IgG3-κ
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About This Item
Conjugate:
unconjugated
Species reactivity:
-
Application:
—
Citations:
9
Pricing and availability is not currently available.
biological source
human
Quality Segment
conjugate
unconjugated
antibody form
purified immunoglobulin
assay
>90% (microfluidic capillary gel electrophoresis)
shipped in
dry ice
storage temp.
−20°C
General description
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. IgG3 is the has a unique extended hinge region conferring greater flexibility
Human myeloma IgG3, κ is purified from human plasma by fractionation, ion-exchange, and affinity chromatography procedures.
Human myeloma IgG3, κ is purified from human plasma by fractionation, ion-exchange, and affinity chromatography procedures.
Application
IgG3, kappa from human myeloma plasma has been used to determine the trisulfide levels in commercial therapeutic IgG1 antibodies and in human myeloma IgG1, IgG2, IgG3 and IgG4 antibodies.
The purified IgG3, κ may be used as an immunoglobulin calibrator, reference antigen, blocking agent or coating protein in a variety of immunoassays including ELISA, dot-blot immunobinding, Western immunoblotting, immunodiffusion, immunoelectrophoresis, hemagglutination, and cell-binding assays. Human myeloma IgG3 was for in flow cytometry in CHO cells.
Physical form
Solution in 20 mM tris buffered saline, pH 8.0.
Analysis Note
The purity and identity are determined by immunoelectrophoresis, indirect ELISA and SDS-PAGE.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| description purified immunoglobulin, >90% (microfluidic capillary gel electrophoresis) | description clone DX, purified immunoglobulin, buffered aqueous solution | description purified immunoglobulin, >90% (microfluidic capillary gel electrophoresis) | description purified immunoglobulin, >95% (SDS-PAGE) |
| antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified immunoglobulin |
| biological source human | biological source mouse | biological source human | biological source human |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| assay >90% (microfluidic capillary gel electrophoresis) | assay ≥95% (microfluidic capillary gel electrophoresis) | assay >90% (microfluidic capillary gel electrophoresis) | assay >95% (SDS-PAGE) |
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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What is the molecular weight of the Human IgG3 product I5654
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