I4144

IL-3 mouse

≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): IL-3, mIL-3, Interleukin-3 from mouse
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD00164817
• Biological source: mouse
• Recombinant: expressed in E. coli
• Assay: ≥98% (SDS-PAGE)
• Form: lyophilized powder
• Mol wt: 15.1 kDa
• Impurities: ≤1.000 EU/μg

Properties

• Product Name: Interleukin-3 from mouse, IL-3, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• biological source: mouse
• Quality Segment: 200
• recombinant: expressed in E. coli
• assay: ≥98% (SDS-PAGE)
• form: lyophilized powder
• potency: 0.01-0.1 ng/mL ED₅₀/EC₅₀
• quality: endotoxin tested
• mol wt: 15.1 kDa
• packaging: pkg of 10 and 50 μg
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1.000 EU/μg
• color: white to yellow
• UniProt accession no.: P01586
• storage temp.: −20°C

Description

General description

Recombinant mouse Interleukin‑3 (IL‑3) is a hematopoietic cytokine expressed in E. coli and supplied as a lyophilized powder. IL‑3 is widely used in mammalian cell culture research involving hematopoietic and immune‑related cellular response studies in vitro.

Application

Recombinant mouse IL‑3 has been used as a supplement in mammalian cell culture media, including RPMI‑based systems, and in in vitro studies of immune and hematopoietic signaling pathways.

Biochem/physiol Actions

Interleukin‑3 (IL‑3) is a multifunctional cytokine that supports proliferation and differentiation of hematopoietic progenitor cells and has been extensively studied in in vitro cell‑based research models.

Interleukin-3 (IL-3) is a multifunctional protein, originally called colony forming unit-stimulating activity (CFU-SA), and is produced by activated T lymphocytes. IL-3 supports the formation of multilineage colonies in the early development of multipotent hematopoietic progenitor cells. IL-3 has been shown to induce colony formation of macrophages, neutrophils, mast cells, and megakaryocytes from agar-suspended bone marrow cells. IL-3 also interacts with IL-2 to stimulate growth of T lymphocytes and to induce IgG secretion from activated B cells. Other synonyms for IL-3 include pan-specific hemopoietin, multicolony stimulating factor, mast cell growth factor, burst promoting activity, histamine-producing cell stimulating factor, P cell stimulating factor, and WEHI-3 factor.

Physical form

Lyophilized with 0.5 mg of bovine serum albumin. Sterile filtered through a 0.2 μm filter.

Analysis Note

The biological activity is measured by the dose-dependent stimulation of the proliferation of mouse M-NFS-60 cells.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)