I0523

IL-2 mouse

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): Interleukin-2, T-cell growth factor, mIL-2, IL-2
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD00164816
• Biological source: mouse
• Recombinant: expressed in E. coli
• Assay: ≥97% (SDS-PAGE)
• Form: lyophilized powder
• Mol wt: 17.2 kDa
• Impurities: ≤1.000 EU/μg

Properties

• Product Name: Interleukin-2 from mouse, IL-2, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• biological source: mouse
• Quality Segment: 200
• recombinant: expressed in E. coli
• assay: ≥97% (SDS-PAGE)
• form: lyophilized powder
• potency: ≤0.2 ng/mL ED₅₀
• quality: endotoxin tested
• mol wt: 17.2 kDa
• packaging: pkg of 5X20 μg, pkg of 20 μg
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1.000 EU/μg
• color: white
• UniProt accession no.: P04351
• storage temp.: −20°C

Description

General description

Recombinant mouse Interleukin‑2 (IL‑2) is an immunomodulatory cytokine expressed in Escherichia coli and supplied as a lyophilized powder. IL‑2 is commonly used in mammalian cell culture research involving immune cell growth and activation studies in vitro.

Application

Recombinant mouse IL‑2 has been used in mammalian cell culture research applications, including lymphocyte culture, immune cell activation, and cytokine‑response studies in vitro.

Biochem/physiol Actions

Interleukin‑2 (IL‑2) is a cytokine that supports proliferation and maintenance of activated T cells and related cell types. IL-2 participates in T cell proliferation and differentiation and controls the homeostasis of T cell. It has been extensively studied in in vitro immune‑related cell culture models.

Physical form

Lyophilized from a 0.2 μm filtered solution in 10 mM sodium citrate, pH 4.0.

Analysis Note

Activity was determined by dose-dependent stimulation of murine CTLL-2 cells. ED₅₀ : ≤ 0.2 ng/ml (corresponds to a specific activity of 5 × 10⁶ units/mg)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)