Skip to Content

Key Documents

View All Documentation

H9408

β-Hydroxybutyrate Dehydrogenase from Pseudomonas lemoignei

lyophilized powder, ≥200 units/mg protein

Synonym(s):

(R)-3-Hydroxybutanoate:NAD+ oxidoreductase, 3-HBDH

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

CAS Number:
9028-38-0
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
1.1.1.30 (BRENDA, IUBMB)
MDL number:
MFCD00130633
Specific activity:
≥200 units/mg protein
Biological source:
bacterial (Pseudomonas lemoignei)
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

bacterial (Pseudomonas lemoignei)

Quality Segment

form

lyophilized powder

specific activity

≥200 units/mg protein

purified by

chromatography

foreign activity

lactic dehydrogenase ≤0.05%, malic dehydrogenase ≤0.1%

storage temp.

−20°C

General description

D-β-hydroxybutyrate dehydrogenase (BDH) is a membrane bound lipid-requiring enzyme.

Application

Suitable for the determination of acetoacetate and D(-)-3-hydroxybutyrate by the method of Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974).
β-Hydroxybutyrate Dehydrogenase from Pseudomonas lemoignei has been used in coupled assay to confirm the formation of acetoacetate.

Biochem/physiol Actions

In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Pseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).
In Paracoccus denitrificans, 3-hydroxybutyrate dehydrogenase plays a key role in the degradation of intracellular polyhydroxybutyrate and polyhydroxyvalerate.

Physical form

lyophilized powder containing sucrose, β-NAD and Tris buffer salts

Other Notes

One unit will oxidize 1.0 μmole of D-β-hydroxybutyrate to acetoacetate per min at pH 7.8 at 37 °C.

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library