H7039

3-Hydroxy-3-methylglutaryl-CoA reductase human

solution

• Synonym(s): HMGCoA Reductase, HMGR
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 1.1.1.34 (BRENDA, IUBMB)
• Specific activity: 2-8 units/mg protein
• Assay: ≥90% (SDS-PAGE)

Properties

• assay: ≥90% (SDS-PAGE)
• Quality Segment: 200
• form: solution
• specific activity: 2-8 units/mg protein
• mol wt: ~76 kDa (for the fusion protein)
• UniProt accession no.: P04035
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... HMGCR(3156)

Description

General description

Consists of the HMG CoA reductase catalytic domain fused to GST protein.

Application

3-Hydroxy-3-methylglutaryl-CoA reductase human has been used:

• in anti-HMGCR enzyme linked immunosorbent assay (ELISA)
• as an antigen to measure anti-HMGCR antibodies by blot analysis
• in HMG-CoA reductase activity assays
• as an antigen to compare in-house antigen via sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE)/western blot

Biochem/physiol Actions

Hydroxy-3-methylglutaryl-CoA reductase catalyzes the committed step in cholesterol biosynthesis.

3-Hydroxy-3-methylglutaryl-CoA reductase/HMGCR serves as a rate limiting enzyme in mevalonate pathway. It also controls the oncogenic Hippo transducers TAZ/YAP. HMGCR also perform as a genetic modifier for Alzheimer′s disease (AD).

Physical form

Supplied as a solution containing 250 μg protein in 50 mM Tris pH 7.5, with 5 mM DTT, 1:200 Protease Inhibitor Cocktail (Catalog Number P8340) and 50% (w/v) glycerol.

Other Notes

One unit will convert 1.0 μmole of NADPH to NADP⁺ per minute at 37 °C.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable