H0160

HIV-1 gag

≥95% (SDS-PAGE), recombinant, expressed in E. coli, Galactosidase-tagged

• Synonym(s): p24 HIV antigen
• UNSPSC Code: 12352204
• NACRES: NA.83
• MDL number: MFCD01635027
• Assay: ≥95% (SDS-PAGE)
• Recombinant: expressed in E. coli
• Mol wt: 39 kDa (plus 114 kDa β-Gal tag)

Properties

• recombinant: expressed in E. coli
• Quality Segment: 200
• assay: ≥95% (SDS-PAGE)
• mol wt: 39 kDa (plus 114 kDa β-Gal tag)
• concentration: 1 mg/mL
• UniProt accession no.: P04591
• storage temp.: −20°C
• Gene Information: human immunodeficiency virus 1 ... gag(155030)

Description

General description

Contains amino acid residues [77-436] of HIV-1 gag antigen.

The human immunodeficiency virus 1 (HIV−1) gag antigen is a recombinant 39 kDa protein encoded by the GAG gene of the human immunodeficiency virus (HIV) type I expressed in and isolated from Escherichia coli. It has a β−galactosidase tag (114 kDa) on the N−terminus. HIV-1 Gag exists as monomers or lower-order oligomers in the cytosol. At the plasma membrane, it forms higher-order multimers.

Application

HIV-1 gag has been used in the coculture with an autologous cluster of differentiation T (CD4⁺ T) to study the ability of monocyte-derived dendritic cells (MDC1) to induce human immunodeficiency virus 1 (HIV−1) latency reversal (LR).

Biochem/physiol Actions

Positive control for HIV antibodies.

The human immunodeficiency virus 1 (HIV−1) gag (p24) antigen reacts strongly with HIV positive serum and may be used as a positive control for evaluating antibodies to HIV. At transcription sites, HIV-1 Gag creates ribonucleoprotein complexes with unspliced viral RNA. The HIV-1 Gag protein is accountable for packaging genomic RNA (gRNA) and the assembly of immature viral particles.

Physical form

Solution in 8 M urea, 20 mM Tris-HCl, pH 8.0, 10 mM 2-mercaptoethanol.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable