H0137

HT Media Supplement (50×) Hybri-Max^™

lyophilized powder, suitable for hybridoma, BioXtra

• Synonym(s): HT Supplement, Hypoxanthine-Thymidin (HT) media supplement
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• Product Name: HT Media Supplement (50×) Hybri-Max^™, lyophilized powder, γ-irradiated, BioXtra, suitable for hybridoma
• Quality Segment: 200
• sterility: γ-irradiated
• product line: BioXtra
• form: lyophilized powder
• technique(s): cell culture | hybridoma: suitable
• impurities: endotoxin, tested
• storage temp.: −20°C

Description

Application

HT Media supplement is a cocktail hypoxanthine and thymidine qualified for use in hybridoma selection and cell culture applications. HT Media Supplement (50×) Hybri-Max^™ has been used as a supplement in INS-1 β-cells co-culture medium. It is suitable for use as medium for hybridoma cells and as Dulbecco′s Modified Eagle Medium (DMEM) for HeLa S3 and MTH1-KO cells.

Preparation Note

Reconstitute contents of vial with 10 ml sterile cell culture medium. Stock solution is sufficient to prepare 500 ml medium. Final working concentration: 100 μM hypoxanthine, 16 μM thymidine.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)