GERPN6302

Iodoacetamide

Cytiva RPN6302, pack of 25 g

• NACRES: NA.77
• UNSPSC Code: 12352202

Properties

• form: solid
• feature: wetted part: no
• packaging: pack of 25 g
• manufacturer/tradename: Cytiva RPN6302
• color: Yellowish
• solubility: cold water: soluble (Easily soluble in cold and hot water)
• storage temp.: 2-8°C

Description

General description

Used for the efficient alkylation of thiols while minimizing oxidation of competing thiol pairs in protein samples.

Application

Iodoacetamide has been used:

• as a component in urea-sodium dodecyl sulfate (SDS) buffer for yeast cell lysis in immunoprecipitation assay
• in equilibration of pH gradient strips during the second dimension run in isoelectric focusing
• as a protein alkylation agent

Biochem/physiol Actions

Iodoacetamide (IAA) modifies thiol groups in proteins by S-carboxyamidomethylation. Iodoacetamide is an alkylating agent. IAA is commonly used to analyze the key role of cysteine residues in the catalytic reactions of enzymes. It is also used in the derivatization of cysteine residues in proteins for proteomic analysis. IAA irreversibly inhibits glyceraldehyde-3-phosphate dehydrogenase (GAPDH).

Features and Benefits

• Ultrapure, proteomic-grade iodoacetamide is used for efficient alkylation of thiols while minimizing oxidation of the competing thiol pairs in protein samples.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Codes: H301,H334,H317,H413
• Precautionary Statements: P280 - P273 - P261 - P342 + P311 - P301 + P310 - P405 - P501
• Storage Class: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects