GE27-5140-01
Microspin™ S-400 HR
Cytiva 27-5140-01, pack of 50 columns
Synonym(s):
Microspin S-400 HR
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About This Item
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packaging
pack of 50 columns
manufacturer/tradename
Cytiva 27-5140-01
storage condition
(20% Ethanol)
(TE buffer (10 mM Tris/HCl, 1mM EDTA, pH 7.6))
matrix
allyl dextran and N, N’-methylenebisacrylamide
particle size
25-75 μm
avg. part. size
50 μm
cleaning
2-13
working range
3-11
suitability
suitable for bioprocess medium
storage temp.
2-8°C
General description
MicroSpin™ columns are designed for the rapid purification of nucleic acids for use in a wide range of applications, including desalting, buffer exchange and removal of primers. Good product yield and purity is obtained with sample volumes from 25-100 μl, and from nanogram to milligram quantities of DNA.
Features and Benefits
- illustra™ MicroSpin™ S-400 HR Columns: For rapid purification of PCR products (>200 bp) from unincorporated primers (<32-mers) and nucleotides using spin-column chromatography. - Accommodates 25 to 50 μ for post-PCR cleanup prior to cloning or a second amplification reaction or 51 to 100 μ for all other applications.
- Convenient: Prepacked with Sephacryl® S-400 HR pre-equilibriated in TE buffer.
- Ready to use: Requires less than 4 min from sample application to collection of purified product.
- Tested in nickase, single- and double-stranded exonuclease and RNase assays.
Storage and Stability
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
MicroSpin is a trademark of Cytiva
Sephacryl is a registered trademark of Cytiva
illustra is a trademark of Cytiva
Storage Class Code
12 - Non Combustible Liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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