G8880

Glutaminase from Escherichia coli

Grade V, lyophilized powder, ≥50 units/mg protein

• Synonym(s): L-Glutamine amidohydrolase
• CAS Number: 9001-47-2
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 3.5.1.2 (BRENDA, IUBMB)
• MDL number: MFCD00131193
• Specific activity: ≥50 units/mg protein
• Biological source: Escherichia coli

Properties

• biological source: Escherichia coli
• Quality Segment: 200
• type: Grade V
• form: lyophilized powder
• specific activity: ≥50 units/mg protein
• mol wt: 110 kDa
• composition: Protein, ~30% biuret
• solubility: water: 1 mg/mL (Clear w/Particle to Sl. Hazy w/Particles)
• cation traces: NH₄⁺: <0.1 μg/unit
• foreign activity: NADH Oxidase ≤0.01%
• storage temp.: −20°C

Description

General description

Glutaminase is an amidohydrolase. It has the catalytic domain in the N-terminal and the C-terminal extension possesses STAS domain.

Application

Glutaminase from E.coli has been used:

• for the determination of glutamine from the cerebral cortex tissue extract
• for immobilization on aminopropylsilylated controlled pore glass (CPG) for determination of glutamine
• in combination with acivicin in in vitro melanoma cells and MCF-7 and OAW-42 cells for cell proliferation and invasiveness assay.

Biochem/physiol Actions

Glutaminase from Escherichia coli along with acivicin, a glutamine analog regulates proliferation and invasiveness of cancer cells. Bacterial glutaminase is implicated in increasing the life span of cancer patients by lowering the tumor burden.

Glutaminase catalyzes the conversion of glutamine to glutamate.

Physical form

Lyophilized powder containing potassium succinate and EDTA

Other Notes

One unit will deaminate 1.0 μmole of L-glutamine per min at pH 4.9 at 37 °C.

Safety Information

• Storage Class: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)