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G7781

Anti-Glutathione-S-Transferase (GST) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-Glutathione-S-Transferase, Anti GST Antibody - Anti-Glutathione-S-Transferase (GST) antibody produced in rabbit, Anti Gst Antibody, Anti-Gst Antibody, Gst Antibody, Anti-GST

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About This Item

NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
DB, ELISA (i), WB
Citations:
192
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biological source

rabbit

Quality Segment

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

packaging

antibody small pack of 25 μL

technique(s)

dot blot: suitable, indirect ELISA: 1:10,000, western blot: 1:2,000 using lysates of induced Escherichia coli expressing GST

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Anti-Glutathione-S-Transferase (GST) antibody produced in rabbit is specific for native as well as denatured-reduced forms of glutathione-S-transferase from Schistosoma japonicum. The soluble GST is encoded by eight different genes in humans namely, α, μ, θ, π, ζ, σ, κ and ω on chromosome 6, 1, 22, 11, 14, 4, not known and 10 respectively.
The antibody is specific for native as well as denatured-reduced forms of glutathione-S-transferase from Schistosoma japonicum. Anti-GST may be used in various immunoassays to identify the expression of GST fusion proteins.

Immunogen

recombinant GST from Schistosoma japonicum expressed in E. coli.

Application

Anti-Glutathione-S-Transferase (GST) antibody produced in rabbit has been used in immunoblotting.

Biochem/physiol Actions

Glutathione-S-transferase (GST) catalyzes the detoxication of electrophiles by glutathione conjugation. This enzyme provides protection against oxidative stress and toxic foreign chemicals. Polymorphism in GST gene is associated with the development of asthma and cutaneous basal cell carcinoma.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
G1160SAB4200237SAB4501874
description

IgG fraction of antiserum, buffered aqueous solution

description

clone GST-2, ascites fluid

description

clone 2H3-D10, purified from hybridoma cell culture

description

affinity isolated antibody

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

biological source

rabbit

biological source

mouse

biological source

mouse

biological source

rabbit

antibody form

IgG fraction of antiserum

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

clone

polyclonal

clone

GST-2, monoclonal

clone

2H3-D10, monoclonal

clone

polyclonal

technique(s)

indirect ELISA: 1:10,000, western blot: 1:2,000 using lysates of induced Escherichia coli expressing GST, dot blot: suitable

technique(s)

dot blot: suitable, western blot: 1:1,000 using purified recombinant GST or lysate of induced bacteria expressing recombinant GST, indirect ELISA: suitable

technique(s)

western blot: 0.2-0.4 μg/mL using detection limit for GST-fustion protein is ∼ 10ng/lane

technique(s)

ELISA: 1:10000, western blot: 1:500-1:1000


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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter



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