G7400

Galactose Oxidase from Dactylium dendroides

lyophilized powder, ≥3,000 units/g solid

• Synonym(s): D-Galactose:oxygen 6-oxidoreductase
• CAS Number: 9028-79-9
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-843-7
• MDL number: MFCD00131118
• EC Number: 1.1.3.9 (BRENDA, IUBMB)
• Specific activity: ≥3,000 units/g solid
• Biological source: fungus (Dactylium dendroides)

Properties

• biological source: fungus (Dactylium dendroides)
• Quality Segment: 200
• form: lyophilized powder
• specific activity: ≥3,000 units/g solid
• storage temp.: −20°C

Description

General description

Galactose oxidase is an extracellular copper-containing enzyme, secreted by the deuteromycete fungus Dactylium dendroides. It catalyzes the oxidation of a range of primary alcohols, including D-galactose, to the corresponding aldehyde, with reduction of oxygen to hydrogen peroxide.

Application

Galactose oxidase may be used as an analytical tool for the specific determination of D-galactose in blood plasma, plant extracts, and phospholipids. It could be used for the characterization of terminal D-galactoside units in several polymers.

Biochem/physiol Actions

Galactose oxidase catalyzes the coversion of D-galactose to D-galacto-hexodialdose.
2-Deoxy-D-galactose, lactose, melibiose, raffinose and stachyose react with galactose oxidase in the peroxidase:o-tolidine system.
Essentially no oxidation of D-glucose, L-galactose, L-arabinose or D-glucuronate has been observed.

The specificity for galactose and other sugars is similar to that described by Avigad.

Other Notes

One unit will produce a ΔA₄₂₅ of 1.0 per min at pH 6.0 at 25 °C, in a peroxidase and o-tolidine system. Reaction volume = 3.4 mL. Light path = 1 cm.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable