G2904

Monoclonal Anti-Goat/Sheep IgG antibody produced in mouse

clone GT-34, ascites fluid

• Synonym(s): Monoclonal Anti-Goat/Sheep IgG
• NACRES: NA.46
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: GT-34, monoclonal
• Application: ELISA (i)
• Citations: 11

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: secondary antibodies
• clone: GT-34, monoclonal
• contains: 15 mM sodium azide
• species reactivity: sheep, goat, bovine
• should not react with: rat, canine, mouse, rabbit, guinea pig, chicken, horse, pig, feline, human
• technique(s): indirect ELISA: 1:10,000-1:20,000
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

IgG is the main type of antibody in human serum, secreted by the B cells. It is found in blood. IgG consists of four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, which are linked by inter-chain disulfide bonds. IgG contains four isotypes IgG1, IgG2, IgG3 and IgG4.

The antibody reacts with an epitope located on the heavy chain of goat IgG1 and IgG2.

Application

Monoclonal Anti-Goat/Sheep IgG antibody produced in mouse has been used in:

• enzyme linked immunosorbent assay (ELISA)
• immunoblotting
• immunoprecipitation
• immunoelectrophoresis

Biochem/physiol Actions

IgG stimulates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a crucial role in antibody-dependent cell-mediated cytotoxicity (ADCC). IgG might be associated with allergy. It has the longest serum half life.

Monoclonal Anti-Goat/Sheep IgG recognizes an epitope located on the heavy chain of goat IgG1 and IgG2. The antibody shows strong cross-reactivity with sheep IgG and weak cross-reactivity with bovine IgG. No cross-reactivity is observed with IgG derived from human, guinea pig, rat, horse, dog, chicken, pig, mouse, rabbit, or cat.

Physical form

The product is provided as ascites fluid with 0.1% sodium azide as preservative.

Preparation Note

For continuous use, store at 2–8 °C for up to one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable