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G2025

Glycerol

greener alternative

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for electrophoresis, ≥99% (GC)

Synonym(s):

1,2,3-Propanetriol, Glycerin

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About This Item

Linear Formula:
HOCH2CH(OH)CH2OH
CAS Number:
56-81-5
Molecular Weight:
92.09
UNSPSC Code:
50161901
NACRES:
NA.21
PubChem Substance ID:
24895082
EC Number:
200-289-5
Beilstein/REAXYS Number:
635685
MDL number:
MFCD00004722
Assay:
≥99% (GC)
Technique(s):
cell culture | insect: suitable, cell culture | mammalian: suitable
Bp:
182 °C/20 mmHg (lit.)
Vapor pressure:
<1 mmHg ( 20 °C)
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vapor density

3.1 (vs air)

Quality Level

300

vapor pressure

<1 mmHg ( 20 °C)

product line

BioReagent

assay

≥99% (GC)

form

viscous liquid

autoignition temp.

698 °F

greener alternative product characteristics

Safer Solvents and Auxiliaries
Design for Degradation
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable

impurities

≤0.005% Chlorinated compounds, ≤0.005% Sulfate (SO4)

refractive index

n20/D 1.474 (lit.)

pH

5.5-8

bp

182 °C/20 mmHg (lit.)

mp

20 °C (lit.)

density

1.25 g/mL (lit.)

suitability

suitable for electrophoresis

greener alternative category

Aligned

SMILES string

OCC(O)CO

InChI

1S/C3H8O3/c4-1-3(6)2-5/h3-6H,1-2H2

InChI key

PEDCQBHIVMGVHV-UHFFFAOYSA-N

General description

Glycerol is odourless, colorless, viscous in nature, and exists as a sweet tasting liquid. It can be derived naturally as well as from petrochemical feedstock. Glycerol has a wide variety of applications, and is one of the most valuable and versatile chemical substances in nature. It can be used as an emollient, solvent, sweetening agent, in pharmaceutical formulations, cosmetics, foodstuffs and toiletries. It is very stable and can be easily stored under normal temperature; also it is non-irritating and has no adverse impact to the environment.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of the 12 Principles of Green Chemistry. Glycerol, with its high boiling point, low flammability, and polarity, is a standout choice. As a non-toxic and biodegradable compound, it aligns perfectly with the principles of ′Safer Solvents and Auxiliaries′ and ′Design for Degradation′. Click here for more information.

Application

Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel′s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.

Biochem/physiol Actions

Glycerol has been used as
  • a supplement during cell culture of Mycobacterium tuberculosis and Mycobacterium avium.
  • a fuel during designing enzymatic biofuel cell.
  • a liquid composite matrix with 4-HCCA and 3-amino­quinoline for analysis of neutral and acidic glycans.
  • a matrix for fast atom bombardment MS.
  • may be employed as liquid matrix for the quantification studies by MALDI (Matrix-assisted laser desorption/ionization mass spectrometry) analysis.
Glycerol is hygroscopic in nature and is soluble in water owing to its three hydrophilic alcoholic hydroxyl groups. It can form both inter- and intramolecular hydrogen bonds, making it a very flexible molecule. The physiologic effect of glycerine is due to cell-mediated immunity, increased IgG production and increased histamine release.

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This Item
49770G789349767
Glycerol BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for electrophoresis, &#8805;99% (GC)

Sigma-Aldrich

G2025

Glycerol

Glycerol puriss. p.a., ACS reagent, anhydrous, dist., &#8805;99.5% (GC)

Sigma-Aldrich

49770

Glycerol

Glycerol ACS reagent, &#8805;99.5%

Sigma-Aldrich

G7893

Glycerol

Glycerol BioUltra, Molecular Biology, anhydrous, &#8805;99.5% (GC)

Sigma-Aldrich

49767

Glycerol

assay

≥99% (GC)

assay

≥99.5% (GC)

assay

≥99.5%

assay

≥99.5% (GC)

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

200

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

GC/GC: suitable

form

viscous liquid

form

viscous liquid

form

viscous liquid

form

viscous liquid

density

1.25 g/mL (lit.)

density

1.25 g/mL (lit.)

density

1.25 g/mL (lit.)

density

1.25 g/mL (lit.)

impurities

≤0.005% Chlorinated compounds, ≤0.005% Sulfate (SO4)

impurities

acrolein and glucose, in accordance, substances darkened by H2SO4, in accordance, ≤0.05% fatty acid esters (as butyric acid), ≤0.1% water

impurities

Acrolein and glucose, passes test, Fatty acid esters, passes test, H2SO4, passes test (darkened), Neutrality, passes test, ≤0.003% Chlorinated compounds (as Cl), ≤0.500% Water (H2O)

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

390.2 °F - Pensky-Martens closed cup

flash_point_c

199 °C - Pensky-Martens closed cup

ppe

Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBT5045
SHBS3979
SHBR8854
SHBS2739
SHBR4187

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Related Content

Product Information Sheet - G2025

Product Information Sheet

Shelley L Forrest et al.
The Journal of comparative neurology, 522(17), 3900-3927 (2014-07-22)
Artemin is a member of the glial cell line-derived neurotrophic factor (GDNF) family that has been strongly implicated in development and regeneration of autonomic nerves and modulation of nociception. Whereas other members of this family (GDNF and neurturin) primarily target
T W Chew et al.
Oncogene, 33(21), 2717-2727 (2013-07-03)
RAS and Rho small GTPases are key molecular switches that control cell dynamics, cell growth and tissue development through their distinct signaling pathways. Although much has been learnt about their individual functions in both cell and animal models, the physiological
Benoît Ranchoux et al.
The American journal of pathology, 185(2), 356-371 (2014-12-17)
Pulmonary veno-occlusive disease (PVOD) is an uncommon form of pulmonary hypertension (PH) characterized by progressive obstruction of small pulmonary veins and a dismal prognosis. Limited case series have reported a possible association between different chemotherapeutic agents and PVOD. We evaluated
View All Related Papers

Global Trade Item Number

SKUGTIN
G2025-500ML04061838255600
G2025-100ML04061838255587
G2025-1L04061838255594

Questions

1–9 of 9 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. What is the concentration of glycerol (e.g., Product No. G2025)?

    1 answer

    1. Based on a density of 1.261 g/mL for glycerol, and a molecular weight of 92.1 g/mol, the concentration of this liquid is 13.7 M.

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  4. What is the viscosity of glycerol?

    1 answer

    1. The viscosity of glycerol is 648 Centistokes at 20 Deg C (68 Deg F).

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  5. In what solvent is glycerol (e.g., Product No. G2025) most soluble?

    1 answer

    1. Glycerol is miscible with water and alcohol at all concentrations. See: The chemicals encyclopedia published by the Royal Society of Chemistry, 12th ed., Entry# 4493.

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  6. Can I autoclave glycerol (e.g., Product No. G2025) before using it (for example) for cell culture?

    1 answer

    1. Glycerol can be autoclaved for 20 minutes, as is or in a solution, generally at approximately 50%.

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  7. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  8. What is the meaning of "Performance" in your specification sheet for Product No. G2025 (Glycerol)?

    1 answer

    1. The "Performance" test demonstrates that a cell freezing medium prepared using that product maintains the viability of mammalian cells after storing the cells for 5 to 7 days at -196 °C.

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  9. Is Product No. G2025 a sterile glycerol product?

    1 answer

    1. Our quality specifications for this product include cell culture testing, but the product is not sterile as it is shipped.

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