Skip to Content
Merck

Skip To

G100120

Sephadex® G-100

Medium

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Size/SKUAvailabilityPrice

About This Item

CAS Number:
UNSPSC Code:
23151817
NACRES:
NA.56
MDL number:
Pricing and availability is not currently available.
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist


Quality Segment

form

powder

technique(s)

LPLC: suitable

matrix

Agarose

matrix active group

polymer

swelling

1 g swells to 15-20 mL gel

bead size

40-120 μm

separation technique

size exclusion (SEC)

General description

Sephadex® is composed of repeating glucose units attached by α-1,6 glucosidic bonds. This structure ensures more aptamer binding sites and greater binding capacity. It is suitable for large-scale purification and is considered stable and can be utilized more than once.
Fractionation Range (MW)
Globular Proteins: 4,000 - 150,000
Dextrans: 1,000 - 100,000

Application

Sephadex®G-100 is a gel filtration medium used in protein chromatography, affinity chromatography and gel filtration chromatography
It has been used:
  • in gel filtration column for α-amylase purification
  • in gel filtration column for the purification of polysaccharidic fraction extracted from the skin of the ray Raja radula
  • to remove excess protamine by size exclusion from cross-linked iron oxide (CLIO)-protamine-linked (B) peptides

Other Notes

G100120-100G′s updated product number is GE17-0060-01

Legal Information

Sephadex is a registered trademark of Cytiva

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
S6147G10050G50150
description

Medium

description

DNA grade, BioReagent, Molecular Biology, medium

description

Superfine

description

Medium

separation technique

size exclusion (SEC)

separation technique

-

separation technique

-

separation technique

size exclusion (SEC)

matrix active group

polymer

matrix active group

phase

matrix active group

phase

matrix active group

polymer

matrix

Agarose

matrix

-

matrix

-

matrix

Agarose

form

powder

form

beads

form

-

form

slurry

technique(s)

LPLC: suitable

technique(s)

buffer exchange: suitable

technique(s)

LPLC: suitable, affinity chromatography: suitable

technique(s)

LPLC: suitable

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

200


Still not finding the right product?

Explore all of our products under Sephadex® G-100


Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)



Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library



Questions

1–3 of 3 Questions  
  1. My Sephadex® G-100 column is cracking. What is the maximum operating pressure that Product G100120 can take?

    1 answer
    1. The maximum operating pressure for this gel is 96 cm water = 1.6 psi.

      Helpful?

  2. What is the maximum flow rate for Product G100120, Sephadex® G-100 columns?

    1 answer
    1. The maximum flow rate is 4.2 ml/min. Data are based on columns of 2.5 cm diameter with a bed height of 30 cm.

      Helpful?

  3. How do I determine which size to use for Product G100120, Sephadex® G-100 columns?

    1 answer
    1. Generally, larger particles offer faster flow rates than finer particles, but poorer resolution.

      Helpful?

Reviews

No rating value

Active Filters