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G0395

β-Glucosidase from almonds

greener alternative

lyophilized powder, ≥2 units/mg solid

Synonym(s):

β-D-Glucoside glucohydrolase

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About This Item

CAS Number:
9001-22-3
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-589-7
MDL number:
MFCD00130628
EC Number:
3.2.1.21 (BRENDA, IUBMB)
Specific activity:
≥2 units/mg solid
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form

lyophilized powder

Quality Segment

300

specific activity

≥2 units/mg solid

mol wt

135 kDa

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

impurities

salt, essentially free

greener alternative category

Enabling,

storage temp.

2-8°C

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles.

Application

β-glucosidase is also used in the synthesis of glucosides and fucosides with various potential applications in pharmaceutical, cosmetic and detergent industries, hydrolytic removal of aglycone moiety from flavonoid and isoflavonoid glycosides, flavor enhancement of fruit juices and wine, and biosynthesis of oligosaccharides.

Biochem/physiol Actions

βglucosidase is involved in the hydrolysis of β-glycosidic bonds connecting carbohydrate residues in β-D-glycosides. They convert cellobiose and cellooligosaccharides produced by the endo and exoglucanases to glucose.
β−glucosidase is involved in the hydrolysis of β−glycosidic bonds connecting carbohydrate residues in β−D-glycosides. It converts cellobiose and cellooligosaccharides produced by the endo and exoglucanases to glucose.

Analysis Note

Crude

Other Notes

One unit will liberate 1.0 μmole of glucose from salicin per min at pH 5.0 at 37 °C.

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This Item
49290G4511A7420
β-Glucosidase from almonds lyophilized powder, ≥2 units/mg solid

Sigma-Aldrich

G0395

β-Glucosidase from almonds

β-Glucosidase from almonds lyophilized, powder, ≥4 U/mg

Sigma-Aldrich

49290

β-Glucosidase from almonds

β-Glucosidase from almonds lyophilized powder, 10-50 units/mg solid

Sigma-Aldrich

G4511

β-Glucosidase from almonds

Amyloglucosidase from Aspergillus niger lyophilized powder, 30-60 units/mg protein (biuret), ≤0.02% glucose

Sigma-Aldrich

A7420

Amyloglucosidase from Aspergillus niger

specific activity

≥2 units/mg solid

specific activity

≥4 U/mg

specific activity

10-50 units/mg solid

specific activity

30-60 units/mg protein (biuret)

form

lyophilized powder

form

powder

form

lyophilized powder

form

lyophilized powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

mol wt

135 kDa

mol wt

Mr ~135000

mol wt

50-75 kDa

mol wt

-

impurities

salt, essentially free

impurities

-

impurities

salt, essentially free

impurities

≤0.02% glucose

Quality Level

300

Quality Level

100

Quality Level

200

Quality Level

200

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Questions

  1. What is the suitable condition of storing stock solutions in 0.1 M NaAc of G0395-2.5KU, β-Glucosidase and the pH value?

    1 answer

    1. For assay purposes, this enzyme is prepared in cold deionized water. The final reaction mix includes 80 mM sodium acetate, 0.8% (w/v) salicin and 1.2 - 2.4 units β-glucosidase. The sodium acetate buffer solution is prepared at 100 mM, pH 5.0. The optimum pH range for this enzyme is 5.0 - 5.5. While ?-Glucosidase is expected to remain stable in 0.1M NaAc, solution stability studies have not been conducted. The enzyme is stable refrigerated for 12 months when prepared in water. The end user would have to determine stability in a sodium acetate buffer. Please refer to the links below for additional information.
      Product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/408/045/g0395pis.pdf
      Enzymatic Activity Assay
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/205/287/g0395enz.pdf

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