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Merck

D9692

Plant Protoplast Digest/Wash Solution

Rapid isolation of viable protoplasts from plant tissue

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About This Item

NACRES:
NA.56
UNSPSC Code:
41105500
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Quality Segment

form

liquid

storage temp.

2-8°C

General description

The Plant Protoplast Digest/Wash Solution is formulated to facilitate the rapid isolation of viable protoplasts from plant tissue. Plant cells are surrounded by a rigid, semi-permeable cell wall composed primarily of three classes of polysaccharides: cellulose, hemicellulose, and pectin. The Plant Protoplast Digest/Wash Solution can be used for the digestion of the cell wall after the addition of enzymes that hydrolyze these polysaccharides, such as cellulase, pectinase, or pectolyase. The Plant Protoplast Digest/Wash Solution can be subsequently used to wash away any remaining hydrolytic enzymes after digestion is complete.

Plant protoplasts are typically used for any of a number of downstream applications. These applications include, but are not limited to, transient gene expression, viral transfection assays, somatic hybridization, electrophysiological studies, and morphological studies.


Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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