CHP1

Imprint^® Chromatin Immunoprecipitation Kit

Complete ChIP reaction in 6 hours in flexible strip well format

• Synonym(s): Chromatin Immunoprecipitation
• NACRES: NA.54
• UNSPSC Code: 41116158

Properties

• Quality Segment: 200
• storage temp.: −20°C

Description

General description

Imprint Chromatin Immunoprecipitation Kit provides a complete solution for Chromatin Immunoprecipitation including columns and reagents for DNA purification and an integrated protocol for ChIP DNA amplification with our GenomePlex^® Whole Genome Amplification Kit (WGA2) . The flexible format allows for immunoprecipitation and purification of DNA from mammalian cells or tissue in a convenient strip-well format.

For Frequently Asked Questions about this kit, please see ChIP Troubleshooting Questions.

Application

The Imprint Chromatin Immunoprecipitation Kit is a flexible, rapid and complete method for investigating protein-DNA interactions by chromatin immunoprecipitation (ChIP).

• Suitable for downstream applications
• Individual target characterization to genome-wide profiling techniques[1][2]
• Characterization of signal transduction pathways
• Verification of ChIp-chIP and ChIP-seq data

Features and Benefits

• Fast—Total protocol time of less than 6 hours making the Imprint kit the fastest on the market
• Sensitive—As few as 10,000 cells required for each ChIP sample
• Convenient—Fewest steps of any available ChIP protocol
• Flexible—Protocols for cells or tissue, and convenient strip-well format for high-throughput applications
• Complete—Includes columns and reagents for DNA purification as well as an integrated protocol for amplification with the GenomePlex technology

Legal Information

GenomePlex is a registered trademark of Takara Bio USA, Inc.

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Also Available Separately

• C2112

  Column Preparation Solution

  SDS
• P4850

  Proteinase K from Tritirachium album, buffered aqueous glycerol solution, Molecular Biology, ≥800 units/mL

  SDS
• P8340

  Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution

  SDS
• M8695

  Normal Mouse IgG antibody produced in mouse

  SDS
• R1530

  Anti-RNA polymerase II from mouse

  SDS

Safety Information

• pictograms: GHS02,GHS08,GHS05,GHS07
• signalword: Danger
• target_organs: Central nervous system
• Storage Class: 3 - Flammable liquids
• flash_point_f: 53.6 °F - closed cup
• flash_point_c: 12 °C - closed cup
• wgk: WGK 3
• hcodes: H225,H290,H302,H315,H319,H334,H336,H412,EUH430
• pcodes: P201 - P202 - P210 - P273 - P391 - P405 - P501
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3