LS 123

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94120801, human colon, Epithelial

• UNSPSC Code: 41106514
• Biological source: human colon
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Modal no. 63 (2n=46), hypotriploid
• Morphology: Epithelial
• Manufacturer/tradename: ECACC

Properties

• biological source: human colon
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 94120801-DNA-5UG, pkg of vial of cells 94120801-1VL
• growth mode: Adherent
• karyotype: Modal no. 63 (2n=46), hypotriploid
• morphology: Epithelial
• products: Carcinoembryonic antigen (CEA)
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Biochem/physiol Actions

Established in 1974 from the second in a series of 3 primary colonic tumours resected from a 65 year old Caucasian female, which was classified as a Duke′s B mucin producing colon adenocarcinoma. LS 123 is aneuploid, fails to grow progressively in nude mice and forms colonies only in enriched semisolid medium developed for tumour stem cells. The cells will grow in either low serum or serum free medium. In chick embryonic skin assays the cells grow as a well differentiated abnormal colonic epithelium with little mitotic activity and low invasiveness. LS 123 exhibits several properties associated with neoplasia, but have little invasive potential and may represent an early stage in tumour development.

Human colon adenocarcinoma

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 9,13
D16S539: 13
D5S818: 11
D7S820: 9
THO1: 7,8
TPOX: 9
vWA: 16

Preparation Note

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) (Heat inactivated).

Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-2x10,000 cells/cm² using trypsin 0.25% or trypsin/EDTA; 5% CO₂, 37°C.

Other Notes

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.