RAMOS Human Lymph, ECACC 85030802

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85030802, human lymph, Lymphoblast

• Synonym(s): RA 1 Cells, Ramos (RA 1) Cells, Ramos 1 Cells, Ramos-RA1 Cells
• UNSPSC Code: 41106514
• eCl@ss: 32160703
• Biological source: human lymph
• Relevant disease(s): cancer
• Growth mode: Suspension
• Karyotype: Modal no. 45
• Morphology: Lymphoblast
• Manufacturer/tradename: ECACC

Properties

• Product Name: RAMOS Cell Line human, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85030802, human lymph, Lymphoblast
• biological source: human lymph
• description: Human Caucasian Burkitt′s lymphoma
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 85030802-DNA-5UG, pkg of vial of cells 85030802-1VL
• growth mode: Suspension
• karyotype: Modal no. 45
• morphology: Lymphoblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Application

Tumourigenicity and virus studies: Epstein-Barr virus (EBV).
RAMOS Cell Line human has been used to carry out a MTT (colorimetric assay) cell viability assay to measure calcium ionophore-induced cell death in Ramos cells under different conditions and to determine the expression of ZFP36 ring finger protein like 1 (ZFP36L1) in Ramos B cells during different stages of B cell differentiation.

Biochem/physiol Actions

Derived from a Burkitt′s lymphoma which does not possess the EBV genome. EBV infectability and permanent conversion into EBV positive sub-lines is possible by in vitro infection. The cells have B lymphocyte characteristics, with surface associated mu and kappa chains.

Human Caucasian Burkitt′s lymphoma

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 13,14
D16S539: 10,13
D5S818: 7,12
D7S820: 11
THO1: 7,9.3
TPOX: 8,9
vWA: 15,16

Preparation Note

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

Disclaimer

This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.