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Merck

C9210

Cyanogen bromide-activated Agarose

lyophilized powder

Synonym(s):

Agarose, cyclic carbonimidate, CNBr-Activated Agarose

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About This Item

CAS Number:
68987-32-6
UNSPSC Code:
41106500
NACRES:
NA.56
MDL number:
MFCD00163385

Key Documents

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form

lyophilized powder

Quality Level

200

technique(s)

affinity chromatography: suitable

matrix

cross-linked 4% beaded agarose

matrix active group

cyanate or related structures

matrix spacer

1 atom (when ligands are coupled via isourea derivative or related linkage)

swelling

1 g swells to 2.5-4.5 mL

capacity

≥10 mg/mL binding capacity (BSA)(via amino groups)

suitability

suitable for chromatography

storage temp.

−20°C

Application

Cyanogen bromide-activated Agarose is lyophilized powder stabilized with lactose used in affinity chromatography, protein chromatography, protein interactions, antibody labeling, antibody modification and attaching antibodies to agarose beads.

Physical form

Lyophilized powder stabilized with lactose

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000512947
0000512947
0000426609
0000426609
0000393638

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DamIP: Using Mutant DNA Adenine Methyltransferase to Study DNA-Protein Interactions <I>In Vivo</I>.
Xiao, R., and Moore, D.D.
Current Protocols in Molecular Biology, 94, 21-21 (2011)
C A Schall et al.
Biotechnology and bioengineering, 53(1), 41-48 (1997-01-05)
The stability of NAD(H) immobilized to a crosslinked agarose support (Sepharose(R)-4B) was examined in buffer solutions at a pH of 7.0 and 8.5. Specifically, this study investigated particle attrition and ligand leakage rates from a cyanogen bromide activated agarose support.
Rodney D Franklin et al.
Obstetrics and gynecology, 101(3), 455-462 (2003-03-15)
To compare the efficacy of unfractionated heparin and low molecular weight heparin in the in vitro binding of antiphospholipid antibodies obtained from the sera of patients with recurrent pregnancy loss. Women with immunoglobulin (Ig) G antibodies to the phospholipids cardiolipin
R A Gilissen et al.
Biochemical pharmacology, 43(12), 2661-2663 (1992-06-23)
A method for the covalent binding of rat liver UDP-glucuronosyltransferase to a cyanogen bromide-activated agarose matrix is described. The rat liver microsomal fraction was solubilized with 8 mM 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS); 90% of the microsomal protein was solubilized. Some 50-60% of
Mi Young Cha et al.
Bioconjugate chemistry, 22(1), 88-94 (2010-12-15)
We developed fluorescent biosensor systems that are either general or selective to fluoroquinolone antibiotics by using a single-chain variable-fragment (scFv) as a recognition element. The selectivity of these biosensors to fluoroquinolone antibiotics was rationally tuned through the structural modification on
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Global Trade Item Number

SKUGTIN
C9210-15G04061833523278
C9210-1G04061833523285
C9210-5G04061833523582

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