C7464

Monoclonal Anti-Cyclin D1 antibody produced in mouse

clone DCS-6, ascites fluid

• MDL number: MFCD00239664
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: DCS-6, monoclonal
• mol wt: antigen 36 kDa (several lower and higher molecular weight bands)
• contains: 15 mM sodium azide
• species reactivity: rat, mouse, human, monkey
• technique(s): immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunohistochemistry (frozen sections): suitable; immunoprecipitation (IP): suitable; microarray: suitable; western blot: 1:200 using a human tumor cell line extract
• isotype: IgG2a
• UniProt accession no.: P24385
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CCND1(595) ; mouse ... Ccnd1(12443) ; rat ... Ccnd1(58919)

Description

General description

Monoclonal Anti-Cyclin D₁ (mouse IgG2a isotype) is derived from the DCS-6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Cyclin D₁ protein is a G₁-phase cyclin in mammalian cells. This protein is a D-type cyclin. The CCD1 gene is located on the human chromosome at 11q13.3.

Immunogen

recombinant human Cyclin D1 protein.

Application

Monoclonal Anti-Cyclin D₁ antibody produced in mouse may be used in:

• immunoblotting[1]
• immunofluorescence[2]
• immunocytochemistry
• immunoprecipitation
• immunohistochemistry[3]
• antibody-mediated knock-out experiments

Biochem/physiol Actions

Cyclin D₁ is involved in regulating cyclin-dependent kinase (CDK) family members. Cyclin D₁ and D₂ are affiliated with CDK4 and/or CDK6 kinase activities that are observed in mid-G₁ and culminate in the late G₁ phase. Amplification of the cyclin D₁ gene is observed in a significant percentage of cancers, including breast, bladder carcinoma, mantle cell lymphomas, head and neck cancers. This gene is also amplified in esophageal squamous cell carcinomas.

Does not cross-react with other D-type cyclins.

Physical form

The product is provided as ascites fluid with 15 mM sodium azide as a preservative.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable