Sephadex C-50 is suitable for larger biomolecules such as proteins in the molecular weight range of 30 to 100 kDa.
C50120
CM Sephadex® C-50
Synonym(s):
CM Sephadex®, Carboxymethyl Sephadex®
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About This Item
Pricing and availability is not currently available.
form
powder
Quality Segment
technique(s)
activity assay: suitable
bead size
40-125 μm (dry)
pore size
~200,000 Da exclusion limit
operating pH
6-10
capacity
4.0-5.0 meq/g ion exchange capacity
compatibility
mode of use weak cation exchange chromatography
General description
C50120-100G′s updated product number is GE17-0220-01
Application
Sephadex C-50 is used for cation exchange media, separation media, resins, ion exchange chromatography, protein chromatography and affinity chromatography. Sephadex® C-50 has been used to study biochemical characteristics of cobra venom and effects of vitamins on lactoperoxidase enzyme activity.
Legal Information
Sephadex is a registered trademark of Cytiva
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This Item | |||
|---|---|---|---|
| pore size ~200,000 Da exclusion limit | pore size ~30,000 Da exclusion limit | pore size ~200,000 Da exclusion limit | pore size - |
| form powder | form powder | form powder | form - |
| capacity 4.0-5.0 meq/g ion exchange capacity | capacity 4.0-5.0 meq/g ion exchange capacity | capacity 3-4 meq/g binding capacity | capacity binding capacity |
| operating pH 6-10 | operating pH 6-10 | operating pH - | operating pH - |
| technique(s) activity assay: suitable | technique(s) activity assay: suitable | technique(s) protein purification: suitable | technique(s) - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level - |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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What molecular weight range is CM Sephadex® C-50, Product C50120, suitable for?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How do I prepare CM Sephadex® C-50, Product C50120?
1 answer-
Weigh out the required amount of dry powder and suspend it in binding buffer. In general, 1 gram of dry powder CM Sephadex C-50 will give about 30 mL final volume, when swollen in saline buffer. Swelling should be done at the pH at which the resin is going to be used. Complete swelling takes 1-2 days at room temperature, or 2 hours at 100°C over boiling water.
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How do I regenerate CM Sephadex® C-50, Product C50120?
1 answer-
Depending on the nature of the sample, regeneration is normally preformed by washing with a high ionic strength buffer (1-2 M NaCl) and/or increasing pH, followed by re-equilibrating in binding buffer.
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