Characterizing the glycosylation pattern of this enzyme is not within the scope of product evaluation in the laboratory. Unfortunately, there is no access to the specific publication containing the details. However, based on the abstract description, the publication "Gene. 1992 Nov 16;121(2):295-304" provides information about the glycosylation patterns of both the dimeric and monomeric forms of recombinant hAChE (rehAChE) expressed in the embryonal kidney 293 cell line, which may be helpful for reference. The PMID for the publication is 1446827.
C1682
Acetylcholinesterase human
recombinant, expressed in HEK 293 cells, lyophilized powder, ≥1,000 units/mg protein (BCA)
Synonym(s):
AChE, Acetylcholine acetylhydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.32
EC Number:
232-559-3
MDL number:
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Product Name
Acetylcholinesterase human, recombinant, expressed in HEK 293 cells, lyophilized powder, ≥1,000 units/mg protein (BCA)
recombinant
expressed in HEK 293 cells
form
lyophilized powder
mol wt
64.6 kDa
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
human ... ACHE(43)
specific activity
≥1,000 units/mg protein (BCA)
1 of 4
This Item | C0663 | SAB4200839 | C2888 |
|---|---|---|---|
| Gene Information human ... ACHE(43) | Gene Information human ... ACHE(43) | Gene Information human ... ACHE(43) | Gene Information - |
| specific activity ≥1,000 units/mg protein (Lowry) | specific activity ≥500 units/mg protein (BCA) | specific activity - | specific activity ≥1,000 units/mg protein |
| recombinant expressed in HEK 293 cells | recombinant - | recombinant - | recombinant - |
| form lyophilized powder | form buffered aqueous solution | form buffered aqueous solution | form lyophilized powder |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| mol wt 64.6 kDa | mol wt ~80 kDa | mol wt - | mol wt 280 kDa |
Analysis Note
The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.
Application
Biochem/physiol Actions
Acetylcholinesterase (AChE) is regarded as a biomarker in neurotoxicity.[4] It is a modulator of nitric oxide signal transduction pathway and marker of membrane integrity and aging.[5] AChE, hydrolyzes choline esters. It terminates the impulse transmission at cholinergic synapses. AChE does this by rapid hydrolysis of the neurotransmitter acetylcholine (ACh) to acetate and choline. AChE inhibitors prevent the cholinesterase enzyme from breaking down ACh and increases the level and duration of the neurotransmitter action.[6]
Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H2O to choline + acetic acid.
General description
Other Notes
One unit will hydrolyze 1.0 μmole of acetylthiocholine to thiocholine and acetate per minute at pH 8.0 at 37 °C.
Physical form
This product is supplied as a lyophilized powder. Lyophilized from 0.22 μm filtered solution in 50mM phosphate buffer pH8.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Agneta Nordberg et al.
Current Alzheimer research, 6(1), 4-14 (2009-02-10)
The current study aimed to compare the effects of different cholinesterase inhibitors on acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) activities and protein levels, in the cerebrospinal fluid (CSF) of Alzheimer disease (AD) patients. AD patients aged 50-85 years were randomized to
Acetylcholinesterase from human erythrocytes as a surrogate biomarker of lead induced neurotoxicity
Gupta VK, et al.
Enzyme Research, 2015 (2015)
Different cholinesterase inhibitor effects on CSF cholinesterases in Alzheimer patients
Nordberg A, et al.
Current Alzheimer Research, 6(1), 4-14 (2009)
Sebastian Oddsson et al.
Molecules (Basel, Switzerland), 25(12) (2020-06-26)
Despite extensive efforts in the development of drugs for complex neurodegenerative diseases, treatment often remains challenging or ineffective, and hence new treatment strategies are necessary. One approach is the design of multi-target drugs, which can potentially address the complex nature
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Is there information available about the glycosylation pattern of the recombinant human acetylcholinesterase (product number C1682) expressed in HEK 293 cells?
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