C0662

6-Carboxyfluorescein

~97% purity (HPLC), powder

• Synonym(s): 6-FAM, FLUOS
• Empirical Formula (Hill Notation): C₂₁H₁₂O₇
• CAS Number: 3301-79-9
• Molecular Weight: 376.32
• NACRES: NA.47
• PubChem Substance ID: 24892266
• UNSPSC Code: 12171500
• MDL number: MFCD00036873
• Beilstein/REAXYS Number: 54341

Properties

• Product Name: 6-Carboxyfluorescein, ≥96% (HPLC)
• Quality Segment: 200
• assay: ≥96% (HPLC)
• form: powder
• technique(s): titration: suitable
• color: dark yellow to dark orange
• mp: >300 °C (lit.)
• solubility: 0.1 M NaOH: 9.80-10.20 mg/mL, clear to slightly hazy, orange
• λmax: 492 nm at 0.1 N in NaOH
• fluorescence: λ_ex 492 nm; λ_em 517 nm (pH 8.0)
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• SMILES string: OC(=O)c1ccc2C(=O)OC3(c4ccc(O)cc4Oc5cc(O)ccc35)c2c1
• InChI: 1S/C21H12O7/c22-11-2-5-14-17(8-11)27-18-9-12(23)3-6-15(18)21(14)16-7-10(19(24)25)1-4-13(16)20(26)28-21/h1-9,22-23H,(H,24,25)
• InChI key: BZTDTCNHAFUJOG-UHFFFAOYSA-N

Description

Application

6-Carboxyfluorescein has been used to check the plasma membrane integrity in sperm cells.

Fluorescent dye.

Biochem/physiol Actions

6-Carboxyfluorescein diacetate is a membrane permeable compound. It can be hydrolysed by intracellular esterases to form a membrane impermeable bright green fluorescent molecule, 6-carboxyfluorescein.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves