B8049

5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt

tablet

• Synonym(s): X-GlcA, X-glucuronide CHA salt
• Empirical Formula (Hill Notation): C₁₄H₁₃BrClNO₇ · C₆H₁₃N
• CAS Number: 114162-64-0
• Molecular Weight: 521.79
• NACRES: NA.32
• PubChem Substance ID: 24892045
• UNSPSC Code: 12352204
• MDL number: MFCD00058543

Properties

• form: tablet
• Quality Segment: 200
• storage temp.: −20°C
• SMILES string: NC1CCCCC1.O[C@@H]2[C@@H](O)[C@@H](O[C@@H]([C@H]2O)C(O)=O)Oc3c[nH]c4ccc(Br)c(Cl)c34
• InChI: 1S/C14H13BrClNO7.C6H13N/c15-4-1-2-5-7(8(4)16)6(3-17-5)23-14-11(20)9(18)10(19)12(24-14)13(21)22;7-6-4-2-1-3-5-6/h1-3,9-12,14,17-20H,(H,21,22);6H,1-5,7H2/t9-,10-,11+,12-,14+;/m0./s1
• InChI key: JXCKZXHCJOVIAV-CYRSAHDMSA-N

Description

Application

Chromogenic substrate for β-glucuronidase (GUS) gene detection.

5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt has been used as a histochemical reagent in X-GLUC staining.

Biochem/physiol Actions

5-bromo-4-chloro-3-indolyl-β-D-glucuronide sodium salt (X-GlcA) is a colorless substrate. The pre-immobilized X-GlcA can be converted by β-glucuronidase enzyme, synthesised by Escherichia coli.

Preparation Note

Contains 10 mg substrate per tablet.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)