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A9771

Albumin–fluorescein isothiocyanate conjugate

protein bovine

Synonym(s):

FITC-albumin

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About This Item

UNSPSC Code:
12352204
eCl@ss:
42010201
NACRES:
NA.32
MDL number:
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biological source

protein from bovine

Quality Segment

form

powder

extent of labeling

≥7 mol FITCmol albumin

technique(s)

ELISA: suitable

solubility

10 mM Tris pH 7.0: 10 mg/mL

application(s)

detection

storage temp.

2-8°C

General description

Albumin makes up 60% of the plasma protein, which is produced by the liver. It regulates colloid osmotic pressure. Albumin is implicated in the transportation, distribution and metabolism of fatty acids, thyroid hormones and drugs. It reduces weight gain, blocks pulmonary edema, decreases ascites and lowers tissue edema.

Application

Albumin−fluorescein isothiocyanate conjugate has been used:
  • as a control to compare the intracellular delivery efficiency of proteins with or without carrier
  • for the fluorescent visualization of proteins and cells
  • as a blocking agent in ELISA
  • as a chemical for brain perfusion and fixation
  • for in vivo capillary–epithelial permeability experiment
  • for confocal microscopy of right ventricular (RV) vasculature in rats

Preparation Note

Prepared from crystallized and lyophilized albumin (A 4378).

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This Item
A4378A1900F4274
technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

SDS-PAGE: suitable

technique(s)

titration: suitable

solubility

10 mM Tris pH 7.0: 10 mg/mL

solubility

water: soluble (50 mg/ml)

solubility

H2O: soluble 50 mg/mL

solubility

acetone: soluble 10 mg/mL

application(s)

detection

application(s)

-

application(s)

-

application(s)

diagnostic assay manufacturing
hematology
histology

form

powder

form

lyophilized powder

form

lyophilized powder

form

powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100


pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Questions

1–6 of 6 Questions  
  1. Is this albumin "Fatty Acid Free"?

    1 answer
    1. This product is not fatty acid free. Currently, a fatty acid free version of this material is not available.

      Helpful?

  2. Is the conjugate chromophore based 5-fluorescein, 6-fluorescein, or is based on an isomeric (5/6-) mixture?

    1 answer
    1. The chromaphore used to prepare this product is Fluorescein 5-isothiocyanate.

      Helpful?

  3. How is the FITC bound to the albumin in Product A9771, Albumin-fluorescein isothiocyanate conjugate (FITC-albumin)?

    1 answer
    1. The FITC is bound through the epsilon amino group of the lysine amino acid residues. Each lot number will have a varying number of FITC molecules bound.

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  5. What are the excitation and emission maxima for Product A9771, Albumin-fluorescein isothiocyanate conjugate (FITC-albumin)?

    1 answer
    1. The excitation maximum is 495 nm and the emission maximum is 520 nm.

      Helpful?

  6. Is there any free FITC present in Product A9771, Albumin-fluorescein isothiocyanate conjugate?

    1 answer
    1. We would not expect there to be free FITC. The final product is dialyzed against distilled water using dialysis tubing with a molecular weight cutoff of 12,000 prior to it being lyophilized.

      Helpful?

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