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Avidin from egg white

Name: Avidin from egg white
Brand: SIGMA

recombinant, expressed in corn, ≥12 units/mg protein (E1%/280)

Synonym(s):

AVID_CHICK

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About This Item

CAS Number:

1405-69-2

UNSPSC Code:

12352202

EC Number:

215-783-6

NACRES:

NA.56

MDL number:

MFCD00130572

Form:

powder

Assay:

≥80% protein basis (biuret)

Recombinant:

expressed in corn

Mol wt:

glycoprotein 66 kDa, subunit 16 kDa

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Properties

recombinant

expressed in corn

Quality Segment

200

assay

≥80% protein basis (biuret)

form

powder

specific activity

≥12 units/mg protein (E1%/280)

mol wt

glycoprotein 66 kDa, subunit 16 kDa

technique(s)

ELISA: suitable, immunoblotting: suitable, immunoelectrophoresis: suitable

UniProt accession no.

P02701

storage temp.

2-8°C

Gene Information

chicken ... AVD(396260)

Description

General description

Avidin is a homotetrameric glycoprotein found in the egg white of birds, reptiles and amphibians. Each subunit is 16 kDa, singly glycosylated and binds to a molecule of biotin with greater affinity and specificity. Recombinant avidin from corn is similar to avidin from egg white in terms of properties like isoelectric point (pI) and antigenic properties except for the molecular weight. Avidin from corn has low molecular weight than chicken egg-derived avidin. This might be due to less complex glycosylation composition in plants. Commercial production of avidin from corn has certain advantages in terms of availability of greater biomass and avoiding the co-purification of animal virus.

Application

Avidin egg white was used in an assay using functionalized xenon as a biosensor to detect biotin-avidin binding. Egg white was used at 80 nmol.

Avidin forms an extremely strong complex with d-biotin (K_d ~ 10^-15). This activity has made both avidin and biotin extremely useful labels in immunochemical methods of detection and quantitation.[1]

Preparation Note

Affinity purified

Other Notes

One unit will bind 1.0 μg of d-biotin.

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1 of 1

This Item	A7294	A3151	A5378
Sigma-Aldrich A8706 Avidin from egg white	Sigma-Aldrich A7294 Avidin–Alkaline Phosphatase	Sigma-Aldrich A3151 Avidin–Peroxidase	Sigma-Aldrich A5378 Albumin from chicken egg white
technique(s) ELISA: suitable, immunoelectrophoresis: suitable, immunoblotting: suitable	technique(s) direct ELISA: 1:70,000, western blot: 1:150,000-1:300,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per lane	technique(s) direct ELISA: 1:50,000	technique(s) bioconjugation: suitable
recombinant expressed in corn	recombinant -	recombinant -	recombinant -
assay ≥80% protein basis (biuret)	assay -	assay -	assay ≥90% (agarose gel electrophoresis)
mol wt glycoprotein 66 kDa, subunit 16 kDa	mol wt -	mol wt -	mol wt 44287 Da by calculation
form powder	form buffered aqueous solution	form lyophilized powder	form lyophilized powder
Gene Information chicken ... AVD(396260)	Gene Information -	Gene Information -	Gene Information chicken ... OVAL(396058)

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Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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