A7721

JumpStart^™ Taq Antibody

Adds hot-start capabilities to any Taq DNA Polymerase

• Synonym(s): Hot start Taq antibody
• UNSPSC Code: 41106300
• NACRES: NA.55
• MDL number: MFCD02100063
• Conjugate: unconjugated
• Clone: monoclonal
• Application: PCR
• Citations: 4

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• clone: monoclonal
• form: liquid
• feature: hotstart, PCR Additives
• concentration: 1.1 mg/mL
• technique(s): PCR: suitable
• color: colorless
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

JumpStart^™ Taq Antibody provides an efficient method for hot-start PCR. This antibody is a neutralizing monoclonal antibody specific to Taq DNA polymerase. When the antibody is bound to Taq, the enzyme is rendered inactive. At the first denaturation step in thermal cycling, the enzyme-antibody complex dissociates, and the JumpStart Taq Antibody becomes nonfunctional. Once the antibody is denatured, the activity of the Taq DNA polymerase is restored and the enzyme functions normally during the PCR reaction. JumpStart Taq Antibody is commonly used to prevent undesirable amplification products.

Application

JumpStart^™Taq Antibody is suitable for use in:

• cytochrome blocked nucleic acid (LNA) probe assay
• ligation-mediated polymerase chain reaction (LM-PCR)
• in Real-Time quantitative PCR Analysis of viral titer
• PCR amplification of complex genomic or cDNA templates
• to amplify very low-copy-number targets
• for the PCR amplification of many thermal cycles (>35) and multiple primer pairs in the same reaction tube

Packaging

Supplied with dilution buffer.

Preparation Note

Two units of Taq DNA polymerase are inactivated by 1 test of JumpStart Taq Antibody.

Other Notes

JumpStart Taq Antibody, 1.1μg/1μL
Dilution Buffer provided in 1 mL vials

JumpStart^™ Taq Antibody is for R&D use only.Not for drug, household, or other uses.

View more detailed information on JumpStart Taq enzymes at www.sigma-aldrich.com/hotstart.

Legal Information

JumpStart is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves