Unfortunately, the ability of this product to bind to all pig breeds has not been tested.
A5670
Anti-Pig IgG (whole molecule)−Peroxidase antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-Pig Antibodies, Anti-Pig Antibodies - Anti-Pig IgG (whole molecule)-Peroxidase antibody produced in rabbit, Anti-Porcine IgG (whole molecule)−Peroxidase antibody produced in rabbit, Anti-Swine IgG (whole molecule)−Peroxidase antibody produced in rabbit
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About This Item
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
DB, ELISA (d)
Citations:
22
Pricing and availability is not currently available.
biological source
rabbit
Quality Segment
conjugate
peroxidase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
pig
technique(s)
direct ELISA: 1:40,000, dot blot: 1:150,000 (chemiluminescent)
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Immunoglobulin is a glycoprotein, with two heavy chain and two light chain connected by disulfide bond. IgG is a major class of immunoglobulin. It has five subclasses of IgG- IgG1, IgG3, IgG2a and IgG2b. Affinity isolated antibody is obtained from rabbit anti-pig IgG antiserum by immunospecific purification which removes essentially all rabbit serum proteins, including immunoglobulins, which do not specifically bind to pig IgG. Anti-Pig IgG is then conjugated to peroxidase by protein cross linking with 0.2% glutaraldehyde.
Immunogen
purified pig IgG
Application
Anti-Pig IgG (whole molecule)?Peroxidase antibody produced in rabbit has been used in enzyme-linked immunosorbent assay (ELISA) and immunoblotting.
Biochem/physiol Actions
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It mainly helps in immune defense. Pig IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-pig IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Rabbit anti-pig IgG (whole molecule)-peroxidase antibody binds to pig IgG.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT.
Preparation Note
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form whole antiserum | antibody form affinity isolated antibody |
| conjugate peroxidase conjugate | conjugate peroxidase conjugate | conjugate unconjugated | conjugate peroxidase conjugate |
| species reactivity pig | species reactivity guinea pig | species reactivity - | species reactivity bovine |
| biological source rabbit | biological source rabbit | biological source rabbit | biological source rabbit |
| technique(s) direct ELISA: 1:40,000, dot blot: 1:150,000 (chemiluminescent) | technique(s) direct ELISA: 1:50,000-1:80,000, dot blot: 1:50,000-1:100,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400-1:800 | technique(s) indirect ELISA: 1:40,000, quantitative precipitin assay: 2.8 mg/mL | technique(s) direct ELISA: 1:20,000, dot blot: 1:80,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 |
| form buffered aqueous solution | form buffered aqueous solution | form - | form buffered aqueous solution |
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Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Will Anit-Pig IgG have the same ability to bind to antibodies from all pig breeds? Including minipigs, or potbellied pigs?
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