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A4541

Anti-Biotin−Peroxidase antibody produced in goat

Name: Anti-Biotin−Peroxidase antibody produced in goat
Brand: SIGMA

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Biotin, Anti Biotin Hrp

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About This Item

NACRES:

NA.46

UNSPSC Code:

12352203

Conjugate:

peroxidase conjugate

Clone:

polyclonal

Application:

ELISA (d)

Citations:

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Properties

biological source

goat

Quality Segment

200

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:2,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Biotin is an essential vitamin required for cells in living organisms and in cultures. The high binding affinity of biotin to egg white avidin or bacteria-derived streptavidin has been used to design immunoassays for detecting antigen-antibody interactions. Goat anti-biotin-peroxidase antibody can be used in many applications where biotin may be introduced as target label.

Specificity and reactivity are determined by Ouchterlony Double Diffusion and immunoelectrophoresis versus the biotin-carrier protein complex. No reactivity is observed versus the carrier protein.

Immunogen

Biotin-KLH conjugate

Application

Biotin-labeled proteins in U937 and BHK cell lysates were analyzed by western blot using HRP-conjugated goat anti-biotin.

Goat anti-biotin-peroxidase antibody can be used for direct ELISA assays at a dilution of 1:2,000.

In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.

May be substituted for avidin to decrease non-specific binding.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with preservative.

Preparation Note

Prepared by the periodate method described by Wilson, M.B., and Nakane, P.K., in "Immunofluorescence and Related Staining Techniques," Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.