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Azure B

Name: Azure B
Brand: SIGMA

Dye content, ≥80%, certified by the Biological Stain Commission, powder

Synonym(s):

N,N,N′-Trimethylthionin, Azure I, Methyleneazure

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About This Item

Empirical Formula (Hill Notation):

C₁₅H₁₆ClN₃S

CAS Number:

531-55-5

Molecular Weight:

305.83

UNSPSC Code:

12171500

NACRES:

NA.47

PubChem Substance ID:

24890740

EC Number:

208-511-2

MDL number:

MFCD00011935

Colour Index Number:

52010

Beilstein/REAXYS Number:

3922630

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Properties

Product Name

Azure B, certified by the BSC

agency

certified by the BSC

Quality Segment

200

form

powder

composition

Dye content, ≥80%

technique(s)

microbe id | staining: suitable

color

dark green

mp

205-210 °C (dec.) (lit.)

solubility

H₂O: 1 mg/mL

fluorescence

λ_ex 648 in ethanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

C[N+](C)=C(C=C1)C=C(C1=N2)SC3=C2C=CC(NC)=C3.[Cl-]

InChI

1S/C15H15N3S.ClH/c1-16-10-4-6-12-14(8-10)19-15-9-11(18(2)3)5-7-13(15)17-12;/h4-9H,1-3H3;1H

InChI key

DNDJEIWCTMMZBX-UHFFFAOYSA-N

Description

Application

Certified for use in Lillie′s modified Nocht′s method for paraffin sections and the NCCLS method for blood smears.

Azure B has been used for the staining of chromosomes.

Biochem/physiol Actions

Azure B is a cationic dye. It is part of the Romanowsky stain, which is used for the staining of blood films. It is also used for the localization of nucleic acids.

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1 of 1

This Item	227935	198269	861006
Sigma-Aldrich A4043 Azure B	Sigma-Aldrich 227935 Azure B	Sigma-Aldrich 198269 Erythrosin B	Sigma-Aldrich 861006 Eosin B
technique(s) microbe id \| staining: suitable	technique(s) -	technique(s) microbe id \| staining: suitable	technique(s) microbe id \| staining: suitable
fluorescence λ_ex 648 in ethanol	fluorescence -	fluorescence -	fluorescence -
color dark green	color -	color dark red	color -
solubility H₂O: 1 mg/mL	solubility water: 1 mg/mL, blue to very deep blue	solubility H₂O: 1 mg/mL, clear	solubility H₂O: 1 mg/mL, clear, red
application(s) diagnostic assay manufacturing hematology histology	application(s) diagnostic assay manufacturing hematology histology	application(s) diagnostic assay manufacturing hematology histology	application(s) diagnostic assay manufacturing hematology histology
form powder	form powder	form powder	form powder

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pictograms

GHS07

signalword

Warning

hcodes

H302

pcodes

P264 - P270 - P301 + P312 - P501

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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