A2714

Aldolase from rabbit muscle

lyophilized powder, ≥8.0 units/mg protein

• Synonym(s): D-Fructose-1,6-bisphosphate-D-glyceraldehyde-3-phosphate-lyase, Fructose-diphosphate Aldolase
• CAS Number: 9024-52-6
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-781-0
• MDL number: MFCD00130453
• EC Number: 4.1.2.13 (BRENDA, IUBMB)
• Specific activity: ≥8.0 units/mg protein
• Biological source: rabbit muscle

Properties

• biological source: rabbit muscle
• Quality Segment: 300
• form: lyophilized powder
• specific activity: ≥8.0 units/mg protein
• composition: Protein, ≥80% biuret
• foreign activity: glyceraldehyde-3-phosphate dehydrogenase ≤0.03%, lactic dehydrogenase ≤0.03%, phosphoglucose isomerase ≤0.6%, pyruvate kinase ≤0.1%, triosephosphate isomerase ≤0.05%
• storage temp.: −20°C

Description

Application

Aldolase is used to convert fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Aldolase, from rabbit muscle has been used for stereospecific deprotonation at DHAP C3 . Product A2714 is essentially sulfate-free and contains citrate buffer salts.

Biochem/physiol Actions

Aldolase is involved in gluconeogenesis, the Calvin cycle and glycolysis. Aldolase, from rabbit muscle, is a class I aldolase which forms covalent Schiff base intermediates. The active site of aldolase is in the center of the α/β ⁸ barrel fold .

Physical form

Essentially sulfate-free containing citrate buffer salts

Other Notes

One unit will convert 1.0 μmole of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate per min at pH 7.4 at 25 °C.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)