A2237

Phosphatase, Alkaline shrimp

Name: Phosphatase, Alkaline shrimp
Brand: SIGMA

≥900 DEA units/mL, buffered aqueous glycerol solution, recombinant, expressed in proprietary host

Synonym(s):

Phosphatase, Alkaline from shrimp, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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About This Item

CAS Number:

9001-78-9

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

3.1.3.1 (BRENDA, IUBMB)

MDL number:

MFCD00131849

Recombinant:

expressed in proprietary host

Concentration:

≥900 DEA units/mL

Key Documents

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recombinant

expressed in proprietary host

form

buffered aqueous glycerol solution

concentration

≥900 DEA units/mL

foreign activity

RNAse, DNAse, none detected

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

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Proteins & Enzymes

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Show Differences

1 of 4

This Item	P5521	P4069	P8361
Sigma-Aldrich A2237 Phosphatase, Alkaline shrimp	Sigma-Aldrich P5521 Phosphatase, Alkaline from bovine intestinal mucosa	Sigma-Aldrich P4069 Phosphatase, Alkaline from Escherichia coli	Sigma-Aldrich P8361 Phosphatase, Alkaline bovine
form buffered aqueous glycerol solution	form aqueous solution	form buffered aqueous glycerol solution	form solid
concentration ≥900 DEA units/mL	concentration ≥1.0 mg/mL	concentration -	concentration -
recombinant expressed in proprietary host	recombinant -	recombinant -	recombinant expressed in Pichia pastoris
storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C
shipped in dry ice	shipped in -	shipped in -	shipped in -
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

Analysis Note

Protein determined by biuret

Application

Alkaline phosphatase was used to examine its role in the prevention of high-fat-diet-induced metabolic syndrome in mice.[1] It also may be used in pasteurization process for milks used in dairy products.[2]

Biochem/physiol Actions

Alkaline phosphatase is commonly responsible for dephosphorylation of nucleotides, proteins and alkaloids. The enzyme nonspecifically catalyzes the dephosphorylation of 5′-termini DNA and RNA phosphomonoesters reactions but it does not affect diphosphate or triphosphate linkages.

General description

Shrimp alkaline phosphatase is a heat labile, hydrolase enzyme. It is a high specific activity alkaline phosphatase purified from a recombinant source. There are two preferred sites for enzyme activity: 5′ protruding, recessive and blunt 5′-termini. The enzyme is irreversibly heat inactivated at 65°C for 15 minutes.

Other Notes

One unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C.

Physical form

solution in 50% glycerol containing 25 mM Tris-HCl, pH 7.5, 1 mM MgCl₂

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Rigidity percolation uncovers a structural basis for embryonic tissue phase transitions.

Nicoletta I Petridou et al.

Cell, 184(7), 1914-1928 (2021-03-18)

Embryo morphogenesis is impacted by dynamic changes in tissue material properties, which have been proposed to occur via processes akin to phase transitions (PTs). Here, we show that rigidity percolation provides a simple and robust theoretical framework to predict material/structural

Hypomorphic mutations in PGAP2, encoding a GPI-anchor-remodeling protein, cause autosomal-recessive intellectual disability.

Lars Hansen et al.

American journal of human genetics, 92(4), 575-583 (2013-04-09)

PGAP2 encodes a protein involved in remodeling the glycosylphosphatidylinositol (GPI) anchor in the Golgi apparatus. After synthesis in the endoplasmic reticulum (ER), GPI anchors are transferred to the proteins and are remodeled while transported through the Golgi to the cell

Insulin affects dopamine metabolism in Drosophila females under normal and stress conditions.

N V Adonyeva et al.

Doklady. Biochemistry and biophysics, 448, 40-42 (2013-03-13)

[Effects of naringin on proliferation, differentiation and maturation of rat calvarial osteoblasts in vitro].

Yuan-Kun Zhai et al.

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, 38(1), 105-111 (2013-04-20)

To investigate the effects of naringin on the proliferation, differention and maturaion of rat calvarial osteoblasts (ROB). Segregated neonatal SD rat skull, enzyme digestion to obtain ROB. The culture medium was replaced every three days. Serial subcultivation proceeded when cells

Interpreting an isolated raised serum alkaline phosphatase level in an asymptomatic patient.

Kate Elizabeth Shipman et al.

BMJ (Clinical research ed.), 346, f976-f976 (2013-04-05)

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