A1935

Photobiotin acetate salt

For labeling DNA probes for hybridizations

• Synonym(s): Biotin {3-[3-(4-azido-2-nitroanilino)-N-methylpropylamino]propylamide} acetate salt, N-(4-Azido-2-nitrophenyl)-N′-(3-biotinylaminopropyl)-N′-methyl-1,3-propanediamine acetate salt
• Empirical Formula (Hill Notation): C₂₃H₃₅N₉O₄S · C₂H₄O₂
• CAS Number: 96087-38-6
• Molecular Weight: 593.70
• NACRES: NA.52
• PubChem Substance ID: 24890604
• UNSPSC Code: 12352200
• MDL number: MFCD00210085

Properties

• grade: Molecular Biology
• Quality Level: 200
• assay: ≥95%
• form: film or powder
• solubility: H₂O: 10 mg/mL (Stable for at least 5 months if kept frozen and protected from light.)
• storage temp.: −20°C
• SMILES string: CC(O)=O.CN(CCCNC(=O)CCCC[C@@H]1SCC2NC(=O)NC12)CCCNc3ccc(cc3[N+]([O-])=O)N=[N+]=[N-]
• InChI: 1S/C23H35N9O4S.C2H4O2/c1-31(12-4-10-25-17-9-8-16(29-30-24)14-19(17)32(35)36)13-5-11-26-21(33)7-3-2-6-20-22-18(15-37-20)27-23(34)28-22;1-2(3)4/h8-9,14,18,20,22,25H,2-7,10-13,15H2,1H3,(H,26,33)(H2,27,28,34);1H3,(H,3,4)/t18-,20-,22-;/m0./s1
• InChI key: FFBLNTOMOSLSQM-AYEYRVMASA-N

Description

General description

Photobiotin acetate is made up of biotin, which is bound with the help of a charged linker arm to a photoreactive arylazide group.

Application

Photobiotin acetate salt has been used to label DNA probes to perform DNA−DNA hybridization to determine genomic relatedness.

Biochem/physiol Actions

Photobiotin is a photo-activatable analog of biotin used primarily for labeling DNA, RNA and proteins. Probes prepared using photobiotin can detect target DNA as little as 0.5 pg.

Photobiotin acetate is capable of labeling single stranded DNA and linear or supercoiled double-stranded DNA.

Disclaimer

Light sensitive.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)