94506

Tetramethylrhodamine-5-maleimide

suitable for fluorescence, ≥85% (HPLC)

• Empirical Formula (Hill Notation): C₂₈H₂₃N₃O₅
• CAS Number: 174568-67-3
• Molecular Weight: 481.50
• UNSPSC Code: 12352116
• NACRES: NA.32
• MDL number: MFCD01311091

Properties

• Quality Segment: 100
• assay: ≥85% (HPLC)
• form: powder
• solubility: DMSO: soluble, methanol: soluble
• fluorescence: λ_ex 543 nm; λ_em 575 nm in 0.1 M phosphate pH 7.0
• suitability: suitable for fluorescence
• storage temp.: −20°C
• SMILES string: N5(C(=O)C=CC5=O)c1cc(c(cc1)C2=C3CC=C(C=C3Oc4c2ccc(c4)N(C)C)N(C)C)C(=O)O
• InChI: 1S/C28H25N3O5/c1-29(2)16-5-9-20-23(14-16)36-24-15-17(30(3)4)6-10-21(24)27(20)19-8-7-18(13-22(19)28(34)35)31-25(32)11-12-26(31)33/h5-9,11-15H,10H2,1-4H3,(H,34,35)
• InChI key: KGFLZYXDJDOIEE-UHFFFAOYSA-N

Description

Application

Tetramethylrhodamine-5-maleimide has been used:

• to concentrate the eluate and in labeling to create IRE1LDS112C
• to derivatize green fluorescent protein (GFP) on Cys48 for experiments with the extrinsic fluorescent probe
• to incubate the single solvent exposed cys mutant to perform protein labeling

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Labeling reagent for protein thiols for cytofluorescence

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)