94281

Urease from Canavalia ensiformis (Jack bean)

powder, white, ~8 U/mg

• Synonym(s): Jack bean urease, Urea amidohydrolase
• CAS Number: 9002-13-5
• UNSPSC Code: 12352204
• eCl@ss: 32160410
• EC Number: 232-656-0
• NACRES: NA.54
• MDL number: MFCD00070858
• EC Number: 3.5.1.5 (BRENDA, IUBMB)
• Specific activity: ~8 U/mg
• Biological source: Canavalia ensiformis

Properties

• biological source: Canavalia ensiformis
• Quality Segment: 100
• form: powder
• specific activity: ~8 U/mg
• mol wt: M_r ~480000
• color: white
• storage temp.: −20°C

Description

General description

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620

Urease (Ure) enzyme is present in the cytosol. It is active at acidic pH. Urease is present in archaea, bacteria, unicellular eukaryotes and plants.

Application

Urease from Canavalia ensiformis may be used for urea determination of various samples, such as legumes . It may be useful for the detection of pathogens as well as heavy-metal ions.

Biochem/physiol Actions

Urease catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease is involved in nitrogen metabolism and urea degradation. Urease from Canavalia ensiformis binds 2 nickel ions per subunit .

Other Notes

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol urea (Cat. No. 51459) per minute at pH 8.0 and 25°C

Safety Information

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves