80635

PIPES

BioXtra, Molecular Biology, ≥99.5% (T)

• Synonym(s): 1,4-Piperazinediethanesulfonic acid, Piperazine-1,4-bis(2-ethanesulfonic acid), Piperazine-N,N′-bis(2-ethanesulfonic acid)
• Empirical Formula (Hill Notation): C₈H₁₈N₂O₆S₂
• CAS Number: 5625-37-6
• Molecular Weight: 302.37
• UNSPSC Code: 12161700
• NACRES: NA.25
• PubChem Substance ID: 57652837
• EC Number: 227-057-6
• Beilstein/REAXYS Number: 817713
• MDL number: MFCD00006159

Properties

• grade: Molecular Biology
• Quality Segment: 100
• product line: BioXtra
• assay: ≥99.5% (T)
• form: powder
• impurities: DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected
• ign. residue: ≤0.2% (as SO₄)
• loss: ≤0.2% loss on drying, 110 °C
• useful pH range: 6.1-7.5
• pKa (25 °C): 6.8
• mp: >300 °C (lit.)
• solubility: 0.1 M NaOH: 0.1 M at 20 °C, clear, colorless
• anion traces: chloride (Cl^-): ≤200 mg/kg, sulfate (SO₄^2-): ≤50 mg/kg
• cation traces: Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤100 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg
• λ: 0.1 M in 1 M NaOH
• SMILES string: OS(=O)(=O)CCN1CCN(CC1)CCS(O)(=O)=O
• UV absorption: λ: 260 nm A_max: 0.055, λ: 280 nm A_max: 0.040
• InChI: 1S/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16)
• InChI key: IHPYMWDTONKSCO-UHFFFAOYSA-N

Description

Application

• Sensitive Detection of Immunoglobulin G Stability Using in Real-Time Isothermal Differential Scanning Fluorimetry: Determinants of Protein Stability for Antibody-Based Therapeutics.: The use of PIPES buffer in this study was integral to maintaining the stability of immunoglobulin G during differential scanning fluorimetry. This method is crucial for evaluating the stability of antibody-based therapeutics (Moggridge et al., 2017).

Other Notes

Hybrid selection of mRNA - component of prehybridization buffer.

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)