76258
Paraplast Plus®
suitable for electron microscopy, pellets (fast-melting)
Synonym(s):
Paraplast Plus® embedding media, Paraffin – polyisobutylene mixture
Product Name
Paraplast Plus®, suitable for electron microscopy, pellets (fast-melting)
form
pellets (fast-melting)
technique(s)
electron microscopy: suitable
thickness
≤1 μm , short ribbons
≤2 μm , sectioning: continuous ribbons
mp
56-57 °C
application(s)
hematology
histology
storage temp.
2-8°C
Quality Level
Application
Paraffin polyisobutylene mixture is a tissue embedding medium composed of highly purified paraffin and polyisobutylene and a low level of dimethyl sulfoxide (DMSO) to enhance the rate of tissue penetration. Paraffin-polyisobutylene (76258) supports thin wrinkle-free sectioning down to 4 microM with more flexible and continuous ribbons.
Recommended for large specimens and tissue that commonly presents infiltration problems.
Features and Benefits
- Produces minimal tissue compression and wrinkle-free sections, and cuts to 4 μm thickness with excellent ribbon continuity.
- 0.8% DMSO enhances ease of infiltration and sectioning for tissues difficult to process with regular Paraplast.
- Higher melt viscosity and lower permeability to water vapor creates better adhesion and a more pliable, flexible film.
- Optimal infiltration and embedding for high-quality sections.
- Great compression resistance and ribbon continuity.
- Remarkable morphology preservation and distortion-free sections.
General description
Paraplast Plus® is a refined mixture of highly purified paraffin containing complex elastomer of regulated molecular weights plus a small percentage of dimethyl sulfoxide (DMSO) for fast tissue penetration. It produces minimal tissue compression and wrinkle-free sections and cuts to 4 μm thickness with excellent ribbon continuity.
Paraplast® tissue embedding media are made from highly purified wax combined with plastic polymers for tensile strength and elasticity. Paraplast Plus® provides rapid infiltration during processing as well as great compression resistance during sectioning for preserved morphology and thin, distortion-free sections.
Our range of Paraplast products improve the elasticity of brittle, difficult-to-cut tissues making it easier to produce excellent, wrinkle-free sections.
Paraplast® tissue embedding media are made from highly purified wax combined with plastic polymers for tensile strength and elasticity. Paraplast Plus® provides rapid infiltration during processing as well as great compression resistance during sectioning for preserved morphology and thin, distortion-free sections.
Our range of Paraplast products improve the elasticity of brittle, difficult-to-cut tissues making it easier to produce excellent, wrinkle-free sections.
Legal Information
Paraplast is a registered trademark of Leica Biosystems
Paraplast Plus is a registered trademark of Leica Biosystems
Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Ewa Urbańczyk-Wochniak et al.
Cellular & molecular biology letters, 7(1), 7-18 (2002-04-11)
This study describes an effective method of in situ RT-PCR (RT-ISPCR) that was developed to localize gene expression in plant tissues. This RT-PCR technique was performed on sectioned tissues of female buds of the cucumber GY3 inbred line. The CUS1
H O Goyal et al.
The Anatomical record, 250(2), 164-171 (1998-03-07)
Since androgens and/or estrogens must bind with specific receptors in order to elicit a response at the target organ(s), it is important to understand factors that regulate expression of androgen receptors (AR) and estrogen receptors (ER). Hence, the objective of
[Paraplast-plus, a tissue embedding medium that enables sections to stick to the slides without adhesives (author's transl)].
D Arn et al.
Mikroskopie, 31(3-4), 99-106 (1975-06-01)
R S Liem et al.
Acta anatomica, 132(2), 164-168 (1988-01-01)
A method is described for the histochemical detection of horseradish peroxidase in Paraplast Plus embedded brain sections. The procedure uses 150-micron-thick Vibratome-cut slices of glutaraldehyde-paraformaldehyde-fixed brain tissue. Tetramethylbenzidine stabilized by diaminobenzidine/cobalt/H2O2 is used as chromogen. The Vibratome-cut slices are dehydrated
Ye Jin et al.
The Plant cell, 21(7), 2072-2089 (2009-07-04)
Invertase plays multiple pivotal roles in plant development. Thus, its activity must be tightly regulated in vivo. Emerging evidence suggests that a group of small proteins that inhibit invertase activity in vitro appears to exist in a wide variety of
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