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41051

Atto 488

BioReagent, suitable for fluorescence, ≥90% (HPLC)

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352108
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product line

BioReagent

Quality Segment

assay

≥90% (HPLC)

manufacturer/tradename

ATTO-TEC GmbH

λ

in methanol: water (1:1) (with 0.1% perchloric acid)

UV absorption

λ: 501-507 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

General description

Atto 488 is a labeling dye with high molecular absorption (90,000) and quantum yield (0.80) as well as sufficient Stokes shift between excitation and emission maximum. It is optimized for excitation with an argon laser, and is characterized by high photostability.

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 488 is suitable for preparation of fluorescence labeling reagents and the study of its physicochemical properties.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

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727092856244039
suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

assay

≥90% (HPLC)

assay

≥90% (HPLC)

assay

≥90% (HPLC), ≥90% (degree of coupling )

assay

≥80% (TLC)

product line

BioReagent

product line

BioReagent

product line

BioReagent

product line

-

UV absorption

λ: 501-507 nm Amax

UV absorption

λ: 501-507 nm Amax

UV absorption

λ: 501-506 nm Amax

UV absorption

λ: 505-511 nm Amax


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Questions

  1. How should I prepare the stock solution?

    1 answer
    1. This ATTO 488 is the free carboxy form and may be used for any kind of spectroscopy. Stock solutions can be prepared with water or aqueous buffer. Due to the high extinction coefficient and its high quantum yield of fluorescence this product is suitable for high-sensitivity detection including single-molecule work. The dye can be activated at the carboxy group for coupling to bio-molecules. For this purpose, dry and amine free DMF or DMSO must be used to dissolve the dye.

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