Key Documents

View All Documentation

05019

7-Diethylamino-3-[N-(2-maleimidoethyl)carbamoyl]coumarin

Name: 7-Diethylamino-3-[<I>N</I>-(2-maleimidoethyl)carbamoyl]coumarin
Brand: SIGMA

BioReagent, suitable for fluorescence, ≥97.0% (HPLC)

Synonym(s):

MDCC

Select a Size

Change View

About This Item

Empirical Formula (Hill Notation):

C₂₀H₂₁N₃O₅

CAS Number:

156571-46-9

Molecular Weight:

383.40

UNSPSC Code:

12352116

PubChem Substance ID:

329748005

NACRES:

NA.32

MDL number:

MFCD07366642

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

product line

BioReagent

Quality Segment

100

assay

≥97.0% (HPLC)

suitability

suitable for fluorescence

SMILES string

CCN(CC)c1ccc2C=C(C(=O)NCCN3C(=O)C=CC3=O)C(=O)Oc2c1

InChI

1S/C20H21N3O5/c1-3-22(4-2)14-6-5-13-11-15(20(27)28-16(13)12-14)19(26)21-9-10-23-17(24)7-8-18(23)25/h5-8,11-12H,3-4,9-10H2,1-2H3,(H,21,26)

InChI key

IXQPRUQVJIJUEB-UHFFFAOYSA-N

Description

Application

Thiol-reactive probe for protein labelling

7-Diethylamino-3-[N-(2-maleimidoethyl)carbamoyl]coumarin is utilized as a fluoresencent biological sensing device . Used for real-time measurements for the release of inorganic phosphates during enzymatic reaction when MDCC is conjugated to a mutant phosphate-binding protein . Also, utilized for intramolecular fluorescence energy transfer (FRET) experiments .

pictograms

GHS07

signalword

Warning

hcodes

H302

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

A fluorescence-based sensing system for the environmental monitoring of nickel using the nickel binding protein from Escherichia coli.

Lyndon L E Salins et al.

Analytical and bioanalytical chemistry, 372(1), 174-180 (2002-04-10)

A sensing system for nickel based on the nickel binding protein (NBP) from Escherichia coli is shown to be feasible. The versatility of NBP was demonstrated by its use in three different assay formats. When the NBP binds nickel, it

Direct, real-time measurement of rapid inorganic phosphate release using a novel fluorescent probe and its application to actomyosin subfragment 1 ATPase.

M Brune et al.

Biochemistry, 33(27), 8262-8271 (1994-07-12)

A probe has been developed that can rapidly measure micromolar concentrations of inorganic phosphate (Pi), in particular to follow the release of Pi in real time from enzymes such as phosphatases. Its application is described to investigate the mechanism of

A new paradigm for DNA polymerase specificity.

Yu-Chih Tsai et al.

Biochemistry, 45(32), 9675-9687 (2006-08-09)

We show that T7 DNA polymerase exists in three distinct structural states, as reported by a conformationally sensitive fluorophore attached to the recognition (fingers) domain. The conformational change induced by a correct nucleotide commits the substrate to the forward reaction

View All Related Papers

Global Trade Item Number

SKU	GTIN
150215-5G	04061831818727
1483185-10MG	04061833852651
PHR3071-25MG	04065266899955
150215-1G	04061825583037
05019-1MG-F	04061823353243
05019-5MG-F	04061833488553