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P3532

Phenol Red

Bioreagent, Powder

Synonym(s):

Phenolsulfonphthalein

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About This Item

Empirical Formula (Hill Notation):
C19H14O5S
CAS Number:
Molecular Weight:
354.38
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
205-609-7
MDL number:
Beilstein/REAXYS Number:
326470
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Product Name

Phenol Red, powder, BioReagent, suitable for cell culture

product line

BioReagent

Quality Segment

form

powder

technique(s)

cell culture | mammalian: suitable

color

dark red to dark brown

visual transition interval

6.8-8.2, yellow to red

mp

>300 °C

solubility

1 M NaOH: 1 mg/mL

application(s)

diagnostic assay manufacturing

storage temp.

room temp

SMILES string

Oc1ccc(cc1)C2(OS(=O)(=O)c3ccccc23)c4ccc(O)cc4

InChI

1S/C19H14O5S/c20-15-9-5-13(6-10-15)19(14-7-11-16(21)12-8-14)17-3-1-2-4-18(17)25(22,23)24-19/h1-12,20-21H

InChI key

BELBBZDIHDAJOR-UHFFFAOYSA-N

General description

Phenol Red is a phenolsulfonphthalein compound. It is present as a sulfonated hydroxyquinone in weakly acidic aqueous solutions. In alkaline solutions, the dye forms a hydrophilic dianion (a small, red anion and has a larger conjugated system than the yellow hydroxyquinone).

Application

Phenol red is commonly used as a pH indicator in biology and medicine. It is incorporated into microbiological culture media for the identification of Escherichia coli strains. Its clinical diagnostic applications include investigation of gastric and duodenal ulcers and in the assessment of tear function in ophthalmology. Phenol red is also used as a tracer to assess the permeability of the ileal mucosal membrane and to assess bacterial leakage through root-filling materials in dentistry.
Suitable for use as a pH indicator.

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This Item
114529P0290P4758
description

powder, BioReagent, suitable for cell culture

description

ACS reagent

description

0.5%, liquid, sterile-filtered, BioReagent, suitable for cell culture

description

pH indicator dye

technique(s)

cell culture | mammalian: suitable

technique(s)

titration: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

titration: suitable

color

dark red to dark brown

color

-

color

-

color

red to brown

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

diagnostic assay manufacturing
hematology
histology

form

powder

form

powder

form

liquid

form

powder

solubility

1 M NaOH: 1 mg/mL

solubility

water: 0.77 g/L at 100 °C

solubility

-

solubility

water: 1 mg/mL, opaque, dark red

storage temp.

room temp

storage temp.

room temp

storage temp.

-

storage temp.

room temp


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pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves



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Questions

  1. I want to use phenol red power as an pH indicator, how much I need to add to 20mM acetic acid solution prepared by adding acetic acid to dH2O. 

    1 answer
    1. Phenol red is typically used as a pH indicator for neutral solutions. The solubility of this product is tested at 100 mg/mL in 1 N Sodium Hydroxide. The material has not been tested for solubility in acids. A 20 mM solution of Acetic acid is expected to have a pH of approximately 3.5. At a pH of 6.8 or less phenol red in solution is yellow. The visual transition interval of this indicator is 6.8 - 8.2, with anything higher than 8.2 being deep red in color.

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