The limit of detection for this product has not been determined. For assay purposes, this Ninhydrin product is tested for suitability at a concentration of 5 ug in a 10 mg/mL solution of b-Alanine. In addition, the 0.2% Ninhydrin spray reagent has been tested for detection of Glycine, Lysine, and Alanine at 1 ug, 5 ug, and 10 ug in TLC. Please see the link below to review a publication that may be of interest.
https://www.sciencedirect.com/science/article/pii/S0039914012005255
N4876
Ninhydrin
powder, suitable for amino acid detection
Synonym(s):
1,2,3-Indantrione monohydrate, 2,2-Dihydroxy-1,3-indanedione, Trioxohydrindene monohydrate
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About This Item
Empirical Formula (Hill Notation):
C9H6O4
CAS Number:
Molecular Weight:
178.14
NACRES:
NA.21
PubChem Substance ID:
UNSPSC Code:
12352100
EC Number:
207-618-1
MDL number:
Beilstein/REAXYS Number:
1910963
Form:
powder
Pricing and availability is not currently available.
Product Name
Ninhydrin, suitable for amino acid detection
form
powder
Quality Segment
mp
250 °C (dec.) (lit.)
solubility
ethanol: 100 mg/mL, H2O: 50 mg/mL (with sonication)
suitability
suitable for amino acid detection
functional group
hydroxyl, ketone
SMILES string
OC1(O)C(=O)c2ccccc2C1=O
InChI
1S/C9H6O4/c10-7-5-3-1-2-4-6(5)8(11)9(7,12)13/h1-4,12-13H
InChI key
FEMOMIGRRWSMCU-UHFFFAOYSA-N
General description
Ninhydrin is an important building block in organic synthesis and has been employed to prepare a variety of chemicals for complexing and/or sensing metal ions. It is an amino acid-sensitive reagent used for the preparation of Ruhemann′s purple.
Application
Ninhydrin can be used:
- For the detection of free amino groups in amino acids, peptides, and proteins.
- As a ligand for the Cu-catalyzed N-arylation of nitrogen-containing heterocycles with aryl and heteroaryl halides to synthesize N-arylheterocycles.
- As a reactant to synthesize aldols by reacting with active methylene compounds.
Used for the detection of free amino groups in amino acids, peptides and proteins.
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This Item | ||
|---|---|---|
| description suitable for amino acid detection | description ACS reagent | description - |
| solubility ethanol: 100 mg/mL, H2O: 50 mg/mL (with sonication) | solubility H2O: passes test | solubility - |
| Quality Level 200 | Quality Level 200 | Quality Level 100 |
| mp 250 °C (dec.) (lit.) | mp 250 °C (dec.) (lit.) | mp - |
| form powder | form solid | form solid |
| suitability suitable for amino acid detection | suitability passes test for sensitivity to amino acids | suitability - |
| functional group hydroxyl, ketone | functional group ketone, hydroxyl | functional group - |
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Signal Word
Warning
Hazard Codes
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
dust mask type N95 (US), Eyeshields, Gloves
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What is the limit of detection when using the reagent?
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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