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Fast Blue B Salt

Name: Fast Blue B Salt
Brand: SIAL

Dye content ~95 %, Powder

Synonym(s):

o-Dianisidine bis(diazotized) zinc double salt, Azoic Diazo No. 48, DBB, Diazo Blue B, Naphthanil Diazo Blue B

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About This Item

Linear Formula:

C₁₄H₁₂N₄O₂Cl₂ · ZnCl₂

CAS Number:

14263-94-6

Molecular Weight:

475.47

UNSPSC Code:

12171500

NACRES:

NA.47

PubChem Substance ID:

24894113

EC Number:

238-153-2

MDL number:

MFCD00075203

Beilstein/REAXYS Number:

1499067

Colour Index Number:

37235

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Properties

Product Name

Fast Blue B Salt, Dye content ~95 %

description

Technical grade

Quality Segment

200

form

powder

composition

Dye content, ~95%

technique(s)

microbe id | staining: suitable

color

yellow to dark brown

mp

>300 °C (lit.)

solubility

H₂O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.COc1cc(ccc1[N+]#N)-c2ccc([N+]#N)c(OC)c2

InChI

1S/C14H12N4O2.4ClH.Zn/c1-19-13-7-9(3-5-11(13)17-15)10-4-6-12(18-16)14(8-10)20-2;;;;;/h3-8H,1-2H3;4*1H;/q+2;;;;;+2/p-4

InChI key

GPPKNJIWDULNQH-UHFFFAOYSA-J

Description

Application

Fast Blue B has been used in the acid phosphatase test in Clostridium perfringens.
It has been used as a spraying agent in thin layer chromatography for the identification of separated components.
It has also been used for the determination of the lipase activity.

Biochem/physiol Actions

Fast blue B is used for the determination of acetylcholinesterase activity. Acetylcholinesterase converts 1-naphthyl acetate to 1-naphthol, which reacts with fast blue B to form a purple-coloured diazonium dye. Similarly, fast blue B is also used for α- and β-glucosidase activity.

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This Item	F0500	201464	F8761
Sigma-Aldrich D9805 Fast Blue B Salt	Sigma-Aldrich F0500 Fast Blue RR Salt	Sigma-Aldrich 201464 Variamine Blue RT Salt	Sigma-Aldrich F8761 Fast Garnet GBC sulfate salt
description Dye content ~95 %	description crystalline	description -	description diazonium dye
technique(s) microbe id \| staining: suitable	technique(s) microbe id \| staining: suitable	technique(s) -	technique(s) microbe id \| staining: suitable
color yellow to dark brown	color -	color -	color orange to very dark orange, and Orange-Brown to Very Dark Orange-Brown and Red to Very Dark
form powder	form crystalline	form solid	form powder
solubility H₂O: 1 mg/mL	solubility water: 1 mg/mL, clear to hazy, yellow-green	solubility 1 M HCl: 10 mg/mL	solubility H₂O: 5 mg/mL, clear
application(s) diagnostic assay manufacturing hematology histology	application(s) diagnostic assay manufacturing hematology histology	application(s) diagnostic assay manufacturing hematology histology	application(s) diagnostic assay manufacturing hematology histology
Quality Level 200	Quality Level 200	Quality Level -	Quality Level 200